Support vector machine for prediction of siRNA silencing efficacy

In order to assist the design of short interfering ribonucleic acids （siRNA）, 573 non-redundant siRNAs were collected from published literatures and the relationship between siRNAs sequences and RNA interference （RNAi） effect is analyzed by a support vector machine （SVM） based algorithm relied on a basebase correlation （BBC） feature. The results show that the proposed algorithm has the highest area under curve （AUC） value （0. 73） of the receive operating characteristic （ROC） curve and the greatest r value （0. 43） of the Pearson＇s correlation coefficient. This indicates that the proposed algorithm is better than the published algorithms on the collected datasets and that more attention should be paid to the base-base correlation information in future siRNA design.

面向蛋白质功能位点识别的机器学习平台构建

有关蛋白质功能的研究是解析生命奥秘的基础,机器学习技术在该领域已有广泛应用。利用支持向量机(support vectormachine,SVM)方法,构建一个预测蛋白质功能位点的通用平台。该平台先提取非同源蛋白质序列,再对这些序列进行特征编码(包括序列的基本信息、物化特征、结构信息及序列保守性特征等),以编码好的样本作为训练数据,利用SVM进行训练,得到敏感性、特异性、Matthew相关系数、准确率及ROC曲线等评价指标,反复测试,得到评价指标最优的SVM模型后,便可以用来预测蛋白质序列上的功能位点。该平台除了应用在预测蛋白质功能位点之外,还可以应用于疾病相关单核苷酸多态性(SNP)预测分析、预测蛋白质结构域分析、生物分子间的相互作用等。