目的:探讨褪黑素(MT)对噪声应激大鼠胃肠传输功能的影响及可能机制.方法:将♂SD大鼠40只随机分为空白对照组、对照1,2组和实验1,2组,实验组和对照组分别在应激前30min ip MT(15 mg/kg)和等体积生理盐水.对照和实验1组和以56式冲锋枪射...目的:探讨褪黑素(MT)对噪声应激大鼠胃肠传输功能的影响及可能机制.方法:将♂SD大鼠40只随机分为空白对照组、对照1,2组和实验1,2组,实验组和对照组分别在应激前30min ip MT(15 mg/kg)和等体积生理盐水.对照和实验1组和以56式冲锋枪射击时产生的120DB(A)的脉冲噪声,对照和实验1组应激1d,对照和实验2组应激3d,建立噪声应激模型.噪声应激后,观察MT在各组大鼠胃内残留率,小肠推进比,血浆中皮质醇、降钙素基因相关肽(CGRP)和胃动素(MTL)的影响.结果:对照1,2组大鼠的胃残留率(54.52%±4.53%,63.56%±5.51% vs 41.41%±7.74%)、小肠推进比(58.42%±8.77%,67.98%±6.63% vs 41.26%±6.08%),血浆皮质醇(1.99±0.08、2.59±0.09 vs 0.95±0.10),MTL浓度(158.09±10.44.180.09±14.85 vs 124.95±7.83)与空白对照组相比明显升高(P<0.01),其血浆CGRP浓度(125.24±8.5,122.02±10.36)与空白对照组(195.03±7.70)相比显著降低;实验1,2组大鼠的胃残留率(44.53%±8.93%,47.52%±4.98%)、小肠推进比(44.18%±4.36%,46.4%±4.96%),血浆皮质醇(1.27±0.14、1.63±0.09),MTL浓度(128.67±10.25,134.29±18.33)与对照组相比显著降低,其血浆CGRP浓度(191.67±10.13,190.04±11.43)与对照组相比显著升高.结论:MT对噪声引起的大鼠胃肠传输功能紊乱具有保护作用,皮质醇、CGRP和MTL在其发病机制中均起到重要作用.展开更多
The long terminl repeat(LTR) of the bovine foamy virus(BFV) contains the viral promoter,which is responsible for viral gene expression in eukaryotic cells We have demonstrated that BFV LTR linked to the luciferase gen...The long terminl repeat(LTR) of the bovine foamy virus(BFV) contains the viral promoter,which is responsible for viral gene expression in eukaryotic cells We have demonstrated that BFV LTR linked to the luciferase gene can express the enzyme efficiently upon transformation into bacteria Deletion analysis and sequence comparison showed that the BFV LTR has a sequence(from-125 to-90)which is greatly homologous to the model bacteria promoter And the proposed transcriptional starting site is at the thymine of-91 or the cytosine of -92 Besides,being fully functional in E coli,the BFV LTR can also be specifically trans activated by BFV tas gene product,Borf-1 protein The responsive element lies between base -310 and -140,which is in accordance with the responsive region in eukaryotic cells The trans-activation of BFV LTR by Borf 1 protein in bacteria offers a useful system to investigate further the specific interaction between Borf-1 protein with BFV LTR and the mechanism of the trans展开更多
文摘目的:探讨褪黑素(MT)对噪声应激大鼠胃肠传输功能的影响及可能机制.方法:将♂SD大鼠40只随机分为空白对照组、对照1,2组和实验1,2组,实验组和对照组分别在应激前30min ip MT(15 mg/kg)和等体积生理盐水.对照和实验1组和以56式冲锋枪射击时产生的120DB(A)的脉冲噪声,对照和实验1组应激1d,对照和实验2组应激3d,建立噪声应激模型.噪声应激后,观察MT在各组大鼠胃内残留率,小肠推进比,血浆中皮质醇、降钙素基因相关肽(CGRP)和胃动素(MTL)的影响.结果:对照1,2组大鼠的胃残留率(54.52%±4.53%,63.56%±5.51% vs 41.41%±7.74%)、小肠推进比(58.42%±8.77%,67.98%±6.63% vs 41.26%±6.08%),血浆皮质醇(1.99±0.08、2.59±0.09 vs 0.95±0.10),MTL浓度(158.09±10.44.180.09±14.85 vs 124.95±7.83)与空白对照组相比明显升高(P<0.01),其血浆CGRP浓度(125.24±8.5,122.02±10.36)与空白对照组(195.03±7.70)相比显著降低;实验1,2组大鼠的胃残留率(44.53%±8.93%,47.52%±4.98%)、小肠推进比(44.18%±4.36%,46.4%±4.96%),血浆皮质醇(1.27±0.14、1.63±0.09),MTL浓度(128.67±10.25,134.29±18.33)与对照组相比显著降低,其血浆CGRP浓度(191.67±10.13,190.04±11.43)与对照组相比显著升高.结论:MT对噪声引起的大鼠胃肠传输功能紊乱具有保护作用,皮质醇、CGRP和MTL在其发病机制中均起到重要作用.
文摘The long terminl repeat(LTR) of the bovine foamy virus(BFV) contains the viral promoter,which is responsible for viral gene expression in eukaryotic cells We have demonstrated that BFV LTR linked to the luciferase gene can express the enzyme efficiently upon transformation into bacteria Deletion analysis and sequence comparison showed that the BFV LTR has a sequence(from-125 to-90)which is greatly homologous to the model bacteria promoter And the proposed transcriptional starting site is at the thymine of-91 or the cytosine of -92 Besides,being fully functional in E coli,the BFV LTR can also be specifically trans activated by BFV tas gene product,Borf-1 protein The responsive element lies between base -310 and -140,which is in accordance with the responsive region in eukaryotic cells The trans-activation of BFV LTR by Borf 1 protein in bacteria offers a useful system to investigate further the specific interaction between Borf-1 protein with BFV LTR and the mechanism of the trans
