Objective： To observe the effects of different moxibustion times on proteins of transient receptor potential vanilloid 3 （TRPV3） ion channel protein and synovial cell apoptosis in rats with rheumatoid arthritis （R...Objective： To observe the effects of different moxibustion times on proteins of transient receptor potential vanilloid 3 （TRPV3） ion channel protein and synovial cell apoptosis in rats with rheumatoid arthritis （RA）, to provide a new basis for the anti-inflammatory mechanism of moxibustion. Methods： A total of 50 Sprague-Dawley （SD） rats were divided into a normal group, a model group, moxibustion group Ⅰ, moxibustion group Ⅱ and moxibustion group III by complete randomization, with 10 rats in each group. Rats in the normal group were bred routinely, and rats in the model group were also bred routinely after successful modeling. After successful modeling, rats in moxibustion group I, Ⅱ and Ⅲ accepted consecutive moxibustion at Zusanli （ST 36） and Shenshu （BL 23） for 15 d, once a day, respectively 5 min, 20 min and 30 min for each session. The degree of paw edema was observed and recorded. Immunohistochemical assay was used to detect the protein expression of TRPV3 ion channel in dorsal root ganglia and spinal cord dorsal horn. Terminal-deoxynucleoitidyl transferase-mediated nick end labeling （TUNEL） was used to detect apoptotic synovial cell number. Results： At the end of treatment, paw circumference of rats in moxibustion groupⅡ and Ill were significantly reduced as compared with that in the model group （P〈0.05）. TRPV3 ion channel protein expression of dorsal root ganglia and spinal cord dorsal horn was higher in the model group than that in the normal group （P〈0.05）; the TRPV3 ion channel protein expressions of dorsal root ganglia and spinal cord dorsal horn in moxibustion group [[ and []I were higher than that in moxibustion group Ⅰ （P〈0.05）; apoptotic synovial cell number in the model group was larger than that in the normal group （P〈0.05）, and apoptotic synovial cell numbers in moxibustion group Ⅱ and Ⅲ were significantly higher than that in the model group （P〈0.05）. Conclusion： Moxibustion of appropriate time could induce TRPV3 expression, and promote svnovial cell apoptosis.展开更多
Objective To investigate the effect of acupuncture on the addiction memory of rats. Methods A model for heroin re-addicted rats was made by coupling heroin injection in increasing doses on the platform of a Morris wat...Objective To investigate the effect of acupuncture on the addiction memory of rats. Methods A model for heroin re-addicted rats was made by coupling heroin injection in increasing doses on the platform of a Morris water maze with high-frequency sounds cues. The animals were randomly divided into control group, relapse group, acupuncture group, and drug group, with 10 individuals in each group. For the acupuncture group, Baihui （百会 GV 20） and ＂Dazhui＂ （大椎 GV 24） were selected. Morris water maze was used to assess the time elapsed and the distance traveled before a rat reached the platform. The expression levels of A FosB and cAMP response element binding protein （CREB） in hippocampal CA 1 area were assessed with immunohistochemical staining. Results Upon completion of three treatments, the acupuncture group had significantly longer lengths of latency and swimming distance compared to the relapse group （P〈0.05）. Furthermore, the acupuncture group had significantly lowered expression levels of A FosB and CREB compared to the relapse group （P〈0.05）. Conclusion Acupuncture can downregulate A FosB and CREB expression in rats＇ hippocampal area and inhibit the addiction memory.展开更多
基金supported by 2014 Undergraduate Innovation and Entrepreneurship Training Program(No.2014041)National Basic Research Program of China 973 Program(No.2015CB554500)~~
文摘Objective： To observe the effects of different moxibustion times on proteins of transient receptor potential vanilloid 3 （TRPV3） ion channel protein and synovial cell apoptosis in rats with rheumatoid arthritis （RA）, to provide a new basis for the anti-inflammatory mechanism of moxibustion. Methods： A total of 50 Sprague-Dawley （SD） rats were divided into a normal group, a model group, moxibustion group Ⅰ, moxibustion group Ⅱ and moxibustion group III by complete randomization, with 10 rats in each group. Rats in the normal group were bred routinely, and rats in the model group were also bred routinely after successful modeling. After successful modeling, rats in moxibustion group I, Ⅱ and Ⅲ accepted consecutive moxibustion at Zusanli （ST 36） and Shenshu （BL 23） for 15 d, once a day, respectively 5 min, 20 min and 30 min for each session. The degree of paw edema was observed and recorded. Immunohistochemical assay was used to detect the protein expression of TRPV3 ion channel in dorsal root ganglia and spinal cord dorsal horn. Terminal-deoxynucleoitidyl transferase-mediated nick end labeling （TUNEL） was used to detect apoptotic synovial cell number. Results： At the end of treatment, paw circumference of rats in moxibustion groupⅡ and Ill were significantly reduced as compared with that in the model group （P〈0.05）. TRPV3 ion channel protein expression of dorsal root ganglia and spinal cord dorsal horn was higher in the model group than that in the normal group （P〈0.05）; the TRPV3 ion channel protein expressions of dorsal root ganglia and spinal cord dorsal horn in moxibustion group [[ and []I were higher than that in moxibustion group Ⅰ （P〈0.05）; apoptotic synovial cell number in the model group was larger than that in the normal group （P〈0.05）, and apoptotic synovial cell numbers in moxibustion group Ⅱ and Ⅲ were significantly higher than that in the model group （P〈0.05）. Conclusion： Moxibustion of appropriate time could induce TRPV3 expression, and promote svnovial cell apoptosis.
基金Supported by National College Student Innovation Entrepreneurship Training Programs:2014038National Natural Science Foundation of China:81173325
文摘Objective To investigate the effect of acupuncture on the addiction memory of rats. Methods A model for heroin re-addicted rats was made by coupling heroin injection in increasing doses on the platform of a Morris water maze with high-frequency sounds cues. The animals were randomly divided into control group, relapse group, acupuncture group, and drug group, with 10 individuals in each group. For the acupuncture group, Baihui （百会 GV 20） and ＂Dazhui＂ （大椎 GV 24） were selected. Morris water maze was used to assess the time elapsed and the distance traveled before a rat reached the platform. The expression levels of A FosB and cAMP response element binding protein （CREB） in hippocampal CA 1 area were assessed with immunohistochemical staining. Results Upon completion of three treatments, the acupuncture group had significantly longer lengths of latency and swimming distance compared to the relapse group （P〈0.05）. Furthermore, the acupuncture group had significantly lowered expression levels of A FosB and CREB compared to the relapse group （P〈0.05）. Conclusion Acupuncture can downregulate A FosB and CREB expression in rats＇ hippocampal area and inhibit the addiction memory.
