毛细胞白血病细胞表面糖蛋白三聚体的免疫电镜检测

目的 为毛细胞白血病 (hairycellleukemia,HCL)的诊断提供新方法。 方法 采用对毛细胞白血病有高度特异性的单抗B ly 7(CD10 3)对 5例患者毛细胞表面的糖蛋白三聚体 (trimericglycoprotein ,TGP)进行免疫电镜研究。 结果 4例HCL患者毛细胞表达TGP ,镜下显示细胞胞质突起呈现出清晰的黑色边缘 ,与邻近染色阴性的细胞形成明显区别。而 1例伴绒毛淋巴细胞的脾淋巴瘤 (spleniclymphomawithvillouslymphocytes,SLVL)和 6例B淋巴细胞慢性白血病 (B chroniclymphocyticleukemia ,B CLL)均显示阴性。

Immunophenotype and Ultrastructure of B-cell Lymphoproliferative Disorder with Cytoplasmic Projection

To identify the knowledge of rare lymphoproliferative disorder, the clinical and biological features of three kinds of lymphoproliferative disorders with cytoplasmic projections were compared The clinical manifestations, ultrastructure and immunophenotype were analyzed The results showed that hairy cell leukemia (HCL), splenic lymphoma with villous lymphocyte (SLVL) and hairy cell leukemia-variant (HCL-V) had some common characters including splenomegaly, peripheral blood and bone marrow infiltration by villous lymphocyte and B lymphocyte immunophenotype; but these three disorders had specific features respectively It was concluded that overall analysis of clinical and laboratory features might be contributive to the differential diagnosis of these three disorders