Factor IX is an important component in human clotting system.Its deficiency ordysfunction leads to hemophilia B.Recently,with the advance of efficient gene transfertechniques,great progress has been made in the gene t...Factor IX is an important component in human clotting system.Its deficiency ordysfunction leads to hemophilia B.Recently,with the advance of efficient gene transfertechniques,great progress has been made in the gene therapy of hemophilia B.It hasbeen moved from labs to clinics and positive results have been reported.In this study,a double-copy retroviral vector(N2CMVIX)and a recombinant plasmid(pCMVIX),bothcontaining human factor IX cDNA,were constructed.The vectors were transferred into cul-tured rabbit skin fibroblast(RSF).The cells capable of secreting high level of human fac-tor IX protein were embedded in collagen matrix and implanted in rabbits allograft展开更多
文摘Factor IX is an important component in human clotting system.Its deficiency ordysfunction leads to hemophilia B.Recently,with the advance of efficient gene transfertechniques,great progress has been made in the gene therapy of hemophilia B.It hasbeen moved from labs to clinics and positive results have been reported.In this study,a double-copy retroviral vector(N2CMVIX)and a recombinant plasmid(pCMVIX),bothcontaining human factor IX cDNA,were constructed.The vectors were transferred into cul-tured rabbit skin fibroblast(RSF).The cells capable of secreting high level of human fac-tor IX protein were embedded in collagen matrix and implanted in rabbits allograft
