β-环糊精与蛋白质非共价复合物的电喷雾质谱研究

电喷雾质谱(ESI-MS)已经广泛应用于非共价复合物的检测和研究[1,2].ESI是一种极软的离子化过程,它不仅能在不断裂共价键的情况下使分子离子化,而且可以在离子化过程中保持分子间弱的非共价键作用.……

车用柴油关键性能指标的中红外光谱快速筛查

选取具有代表性的、能够覆盖质量指标范围的车用柴油样品集合,即样品集,利用中红外燃油分析仪,以空气为参比,采集样品集背景光谱。利用化学计量学软件,以偏最小二乘法(PLS)建立各项质量指标与光谱数据关系的定标模型,用定标样品集的统计标准偏差(SEC)评价定标模型的准确性,以SEC是否满足参考标准方法的再现性进行评价。使用定标样品集外的样品验证定标模型的准确性,通过与标准检测方法进行对比实验,验证定标模型是否满足标准方法中规定的再现性要求,并进一步用统计学中配对t检验进行评价,验证定标模型是否与标准检测方法存在显著性差异。

Ligand Exchange Between Penta-Coordinated Phosphoryl Serine and Histidine Compounds

With the assistance of HPLC-ESI-MS/MS,the self-assembly products of serine and histidine penta-coordinated phosphorus compound were separated and identified. The expectative product was seryl-histidine dipeptide,but it was found that there was almost equimolar amount of histidyl-histidine dipeptide as well as seryl-histidine dipeptide. The mechanism was speculated that there was ligand exchange between penta-coordinated phosphoryl serine and histidine in the reaction process. As a result,two types of dipeptide were produced.