The nutritional components and poisonous elements in flowers of Camellia chekiangoleosa,C.polydonta,C.semiserrata and C.azalea were studied.The results indicated that the soluble sugar content of the four Camellia flo...The nutritional components and poisonous elements in flowers of Camellia chekiangoleosa,C.polydonta,C.semiserrata and C.azalea were studied.The results indicated that the soluble sugar content of the four Camellia flowers had no difference.The maximum protein content was 82.9 g·kg-1,detected in the flower of C.semiserrata.And the minimum protein content was 56.8 g·kg-1,found in C.azalea.Vitamin C and β-carotene contents in C.azalea and C.semiserrata were higher,and in C.polydonta and C.chekiangoleosa were lower.The total amino acid contents of the four Camellia were 68.41、67.26、50.77、66.87 g·kg-1 respectively,the essential amino acid contents were 33.86、32.70、27.21、33.79 g·kg-1 respectively,and the proportion of essential amino acid in total amino acid of the four Camellia were 49.50%,48.62%,53.59% and 50.53% respectively.The contents of Ca,Mn and Cu in C.chekiangoleosa were the highest.Zn and Se contents were the maximum and Ca was the minimum in C.polydonta.The contents of Fe and Se were the lowest in C.semiserrata.Fe content were the maximum and Mn,Zn and Cu the minimum in C.azalea.The trace element contents in the flower of the four Camellia species followed the order of Mn,Fe,Zn,Cu,Se,but the content of Fe was higher than that of Mn in C.azalea.The contents of Pb,Cd,As and Hg of the four Camellia flower were all lower than the permissible amount.展开更多
Agrobacteriurn tumefaciens-mediated transformation(AtMT) method was used to study the genetic transformation in Rhizopus arrhizus of antisense expression vector pBI121-fad2 of VeFAD2 gene.We studied the key factors in...Agrobacteriurn tumefaciens-mediated transformation(AtMT) method was used to study the genetic transformation in Rhizopus arrhizus of antisense expression vector pBI121-fad2 of VeFAD2 gene.We studied the key factors influencing transformation,such as A.tumefaciens strains,bacterial cell volume initially used,co-cultivation time,effective period and concentration of AS.The results shows that the ideal A.tumefaciens strain is AGL-1,the optimum bacterial cell volume initially used is 50-100 μL and co-culture 24 hours will get the most transformants.At co-culture period,AS induction is indispensable.It is beneficial to improve transformation ratio by adding to 200 μmol·L-1 AS at preincubate period and increasing AS concentration to 400-600 μmol·L-1 at co-culture period.It determined that antisense VeFAD2 gene has been integrated into the genome of R.arrhizus by PCR-Southern detection.展开更多
文摘The nutritional components and poisonous elements in flowers of Camellia chekiangoleosa,C.polydonta,C.semiserrata and C.azalea were studied.The results indicated that the soluble sugar content of the four Camellia flowers had no difference.The maximum protein content was 82.9 g·kg-1,detected in the flower of C.semiserrata.And the minimum protein content was 56.8 g·kg-1,found in C.azalea.Vitamin C and β-carotene contents in C.azalea and C.semiserrata were higher,and in C.polydonta and C.chekiangoleosa were lower.The total amino acid contents of the four Camellia were 68.41、67.26、50.77、66.87 g·kg-1 respectively,the essential amino acid contents were 33.86、32.70、27.21、33.79 g·kg-1 respectively,and the proportion of essential amino acid in total amino acid of the four Camellia were 49.50%,48.62%,53.59% and 50.53% respectively.The contents of Ca,Mn and Cu in C.chekiangoleosa were the highest.Zn and Se contents were the maximum and Ca was the minimum in C.polydonta.The contents of Fe and Se were the lowest in C.semiserrata.Fe content were the maximum and Mn,Zn and Cu the minimum in C.azalea.The trace element contents in the flower of the four Camellia species followed the order of Mn,Fe,Zn,Cu,Se,but the content of Fe was higher than that of Mn in C.azalea.The contents of Pb,Cd,As and Hg of the four Camellia flower were all lower than the permissible amount.
文摘Agrobacteriurn tumefaciens-mediated transformation(AtMT) method was used to study the genetic transformation in Rhizopus arrhizus of antisense expression vector pBI121-fad2 of VeFAD2 gene.We studied the key factors influencing transformation,such as A.tumefaciens strains,bacterial cell volume initially used,co-cultivation time,effective period and concentration of AS.The results shows that the ideal A.tumefaciens strain is AGL-1,the optimum bacterial cell volume initially used is 50-100 μL and co-culture 24 hours will get the most transformants.At co-culture period,AS induction is indispensable.It is beneficial to improve transformation ratio by adding to 200 μmol·L-1 AS at preincubate period and increasing AS concentration to 400-600 μmol·L-1 at co-culture period.It determined that antisense VeFAD2 gene has been integrated into the genome of R.arrhizus by PCR-Southern detection.
