目的:探讨神经母细胞瘤(NB)中酪氨酸激酶受体(TR Ks)和神经营养素(NTs)的基因表达及其意义。方法:采用逆转录聚合酶链反应(RT-PCR)法半定量检测27例N B中TrkA、TrkB、TrkC及BDN F m R NA的表达。结果:TrkA的高表达率及总表达率在低分期...目的:探讨神经母细胞瘤(NB)中酪氨酸激酶受体(TR Ks)和神经营养素(NTs)的基因表达及其意义。方法:采用逆转录聚合酶链反应(RT-PCR)法半定量检测27例N B中TrkA、TrkB、TrkC及BDN F m R NA的表达。结果:TrkA的高表达率及总表达率在低分期组均明显大于高分期组(P<0.05),而其高表达水平与患者2年存活率之间具有明显的相关性(P<0.01)。TrkB的高表达率及总表达率在高分期组均明显大于低分期组(P<0.05),而BD NF的三种表达率在两组间比较则均无显著性差异(P>0.05),但这两者的共同表达率在高分期组则明显大于低分期组(P<0.05)。TrkC的表达通常伴有TrkA的表达,而这两者的共同表达率在低分期组则明显大于高分期组(P<0.05),但它们的表达之间并不存在着关联性趋势。结论:RT-PCR法检测酪氨酸激酶受体和神经营养素的基因表达对NB肿瘤的分期和预后评估具有重要的临床意义。展开更多
To study tyrosine-kinase receptor expression in human neuroblastomas.Methods The expression levels of TrkA,TrkB and TrkC receptors were semiquantitatively examined by SABC immunohistochemistry technique in 40 cases of...To study tyrosine-kinase receptor expression in human neuroblastomas.Methods The expression levels of TrkA,TrkB and TrkC receptors were semiquantitatively examined by SABC immunohistochemistry technique in 40 cases of neuroblastomas.Results TrkA expression was only localized to NB cells in the tumor tissue and correlated positively with low tumor stage and favorable outcome (P<0.01).TrkB expression was primarily localized to spindle cells in the fibrovascular stroma of advanced tumor,but the correlation of TrkB expression with tumor stage and outcome wasn’t statistically significant (P>0.05).TrkC expression was mainly localized to NB cells in the tumor tissue and usually accompanied by TrkA expression in NB cells of developed differentiation,but TrkC expression didn’t statistically correlate with tumor stage and outcome (P>0.05).Conclusion The expression of tyrosine-kinase receptor TrkA correlates with the tumor stage and prognosis in neuroblastomas.10 refs,2 figs,1 tab.展开更多
文摘目的:探讨神经母细胞瘤(NB)中酪氨酸激酶受体(TR Ks)和神经营养素(NTs)的基因表达及其意义。方法:采用逆转录聚合酶链反应(RT-PCR)法半定量检测27例N B中TrkA、TrkB、TrkC及BDN F m R NA的表达。结果:TrkA的高表达率及总表达率在低分期组均明显大于高分期组(P<0.05),而其高表达水平与患者2年存活率之间具有明显的相关性(P<0.01)。TrkB的高表达率及总表达率在高分期组均明显大于低分期组(P<0.05),而BD NF的三种表达率在两组间比较则均无显著性差异(P>0.05),但这两者的共同表达率在高分期组则明显大于低分期组(P<0.05)。TrkC的表达通常伴有TrkA的表达,而这两者的共同表达率在低分期组则明显大于高分期组(P<0.05),但它们的表达之间并不存在着关联性趋势。结论:RT-PCR法检测酪氨酸激酶受体和神经营养素的基因表达对NB肿瘤的分期和预后评估具有重要的临床意义。
文摘To study tyrosine-kinase receptor expression in human neuroblastomas.Methods The expression levels of TrkA,TrkB and TrkC receptors were semiquantitatively examined by SABC immunohistochemistry technique in 40 cases of neuroblastomas.Results TrkA expression was only localized to NB cells in the tumor tissue and correlated positively with low tumor stage and favorable outcome (P<0.01).TrkB expression was primarily localized to spindle cells in the fibrovascular stroma of advanced tumor,but the correlation of TrkB expression with tumor stage and outcome wasn’t statistically significant (P>0.05).TrkC expression was mainly localized to NB cells in the tumor tissue and usually accompanied by TrkA expression in NB cells of developed differentiation,but TrkC expression didn’t statistically correlate with tumor stage and outcome (P>0.05).Conclusion The expression of tyrosine-kinase receptor TrkA correlates with the tumor stage and prognosis in neuroblastomas.10 refs,2 figs,1 tab.
