Every eukaryotic cell is a busy microcosm filled with organelles,and the proper functioning of a protein is contingent upon its arrival at the correct location.As such,transportation of proteins takes place constantly...Every eukaryotic cell is a busy microcosm filled with organelles,and the proper functioning of a protein is contingent upon its arrival at the correct location.As such,transportation of proteins takes place constantly within and between cells,some of which is constitutive and some regulated.For example,fibroblasts secrete collagens constitutively,which involves transportation of newly synthesized collagen proteins from the endoplasmic reticulum to the Golgi apparatus,and finally across the plasma membrane via secretory vesicles[1].In contrast,only under stressful conditions does the transcription factor forkhead box O(FOXO)translocate from the cytoplasm to the nucleus to launch a transcriptional response consisting of hundreds of genes[2–4].展开更多
文摘蛋白质组学的兴起带动了质谱技术的快速发展,而质谱技术的进步则拓宽了蛋白质组学研究问题的广度.最近10年内,肽段或完整蛋白质在质谱仪中的裂解技术——电子捕获裂解(electron capture dissociation,ECD)与电子转运裂解(electron transfer dissociation,ETD)逐渐发展起来.ECD和ETD在蛋白质组学中的应用,特别是在蛋白质的翻译后修饰鉴定和"自顶而下(Top-down)"的完整蛋白质裂解研究中已经展示出了诱人的前景.对ECD和ETD的基本原理、质谱特点、仪器实现、数据解析算法与软件开发,以及在蛋白质组学中的应用进展等方面进行了比较系统全面的阐述,并对当前的研究问题、面临的技术挑战与未来的发展趋势等方面作了深入剖析.
基金the Ministry of Science and Technology of China(2020YFF01014505)the Municipal Government of Beijing(in the form of National Institute of Biological Sciences(NIBS)intramural grants)。
文摘Every eukaryotic cell is a busy microcosm filled with organelles,and the proper functioning of a protein is contingent upon its arrival at the correct location.As such,transportation of proteins takes place constantly within and between cells,some of which is constitutive and some regulated.For example,fibroblasts secrete collagens constitutively,which involves transportation of newly synthesized collagen proteins from the endoplasmic reticulum to the Golgi apparatus,and finally across the plasma membrane via secretory vesicles[1].In contrast,only under stressful conditions does the transcription factor forkhead box O(FOXO)translocate from the cytoplasm to the nucleus to launch a transcriptional response consisting of hundreds of genes[2–4].
