大叶栎人工林木材纤维形态变异研究

[目的]探讨大叶栎人工林木材纤维形态的变异规律。[方法]以大叶栎23年生人工林为材料,测定纤维的长度、宽度、腔径、双壁厚、长宽比、壁腔比,分析大叶栎木材纤维沿树干高度和随年轮的变异规律。[结果]大叶栎人工林木材纤维长度、宽度、腔径、双壁厚和长宽比、壁腔比分别变化在1098.2～1224.6、24.22～26.38、15.95～17.02、8.26～9.36μm和43.37～51.12、0.521～0.555,平均值分别为1156.2、25.43、16.56、8.87μm和46.01、0.540。纤维长度、长宽比和壁腔比随树干高度增加呈下降趋势,随年轮的增加呈增加趋势。[结论]大叶栎人工林木材纤维能满足纤维工业原料的要求,是一种较好的纸浆工业用材。

高效液相色谱法分离和测定油茶茎尖组织中4种内源激素

建立了高效液相色谱法测定油茶茎尖组织中生长素(IAA)、赤霉素(GA3)、玉米核苷(ZR)、脱落酸(ABA)4种内源激素的方法。应用反相C18色谱柱,以甲醇-水(含0.75%冰乙酸,35∶65,V/V)为流动相,流速为0.8 ml/min,进样量10μl,检测波长254 nm,柱温30℃,该方法各峰分离效果理想。IAA、GA3、ZR、ABA的最低检出限分别为0.05、0.01、0.02、0.01 mg/L,回收率分别为98.2%、97.9%、96.3%、93.4%。该方法易操作且准确性高。

Genetic Diversity of Wild and Cultivated Populations of Castanopsis hystrix in China

[Objective] This study aimed to investigate the genetic variation of wild and cultivated populations of Castanopsis hystrix. [Method] Genetic variation of five wild populations and three cultivated populations of Castanopsis hystrix, was investigated with ISSR-PCR amplification. Totally, 151 individuals were selected and analyzed by amplification using nine pairs of ISSR primers screened. [Result] Each primer pair produced 7-20 bands and 122 polymorphic bands were obtained. At population level, ISSR diversity in the wild populations (P=59.84%, HPOP=0.182 7, I=0.285 6) was higher than which in cultivated ones (P=54.87%, HPOP=0.136 6, and I=0.219 8). The genetic differentiation coefficient among wild populations (GST) was 0.99. The similar population structure was found in three cultivated populations (GST=0.127 5). According to the UPGMA cluster analysis, the genetic distance among wild populations became larger with the increase of geographical distance. [Conclusion] Compared with other seed plants, with either a similar life history or various breeding system attributes, relatively low level of genetic diversity was observed in these five wild populations, which was caused by population size reduction and habitat fragmentation related to human activities. The formation of population structure may be explained by the species’ breeding system.

红锥ISSR-PCR扩增体系的优化

对广西红锥种群的DNA进行提取和ISSRPCR扩增体系进行优化。分析了退火温度、模板DNA浓度、Mg2+浓度、dNTPs浓度、TaqDNA聚合酶用量对反应结果的影响。红锥ISSRPCR分析较适宜的扩增体系是:25μL PCR反应体积中,buffer(10 mM TrisHCl,pH 9.0,50 mM KCl,0.1%Triton X100),1.25 U Taq DNA聚合酶,4种dNTPs各0.2 mM,0.4μM引物,2.0mM MgCl2,50 ng模板DNA。

广西国有林场改革与发展的思路

介绍了广西国有林场目前所存在的问题,主要涉及从问题表象出发分析产生问题的根源,并从7个方面对广西国有林场提出改革与发展的思路。这些问题包括:明晰产权问题"、政企分离"问题、分类经营问题、分离第一产业问题、集中力量发展第二产业问题、大力发展林下经济问题。

桉树轻基质组培苗的成本核算与控制

以广西国有维都林场组培厂为例,在年产500万株桉树轻基质组培苗的生产情况下,对组培苗生产、轻基质制作、炼苗及成苗等三个业务分部进行生产成本核算,并分析其成本的控制。结果表明:组培苗生产分部占总成本的42.721%,轻基质制作分部占总成本的27.941%;炼苗及成苗分部占总成本的15.902%;其他占总成本的13.436%。桉树轻基质组培苗主要在操作员工和管理人员方面进行成本控制。