目的:观察跑台运动对高脂饮食诱导的肥胖大鼠骨骼肌毛细血管生成及apelin/APJ表达的影响。方法:5周龄健康雄性SD大鼠随机分成对照组和高脂喂养组,分别进行普通饲料和高脂饲料喂养,12周后筛选肥胖大鼠16只,随机分为高脂安静组和高脂运动...目的:观察跑台运动对高脂饮食诱导的肥胖大鼠骨骼肌毛细血管生成及apelin/APJ表达的影响。方法:5周龄健康雄性SD大鼠随机分成对照组和高脂喂养组,分别进行普通饲料和高脂饲料喂养,12周后筛选肥胖大鼠16只,随机分为高脂安静组和高脂运动组。跑台训练持续10周,每周5次,每次60分钟,跑速26米/分钟。测定大鼠体重、体脂重及血脂水平,免疫组化法观察比目鱼肌毛细血管密度和apelin蛋白表达水平,放免法测定比目鱼肌apelin含量,real-time PCR方法测定比目鱼肌apelin和APJ的m RNA表达。结果:与对照组相比,高脂饮食喂养大鼠体重和体脂显著增加(P<0.01),总甘油三酯、总胆固醇和低密度脂蛋白胆固醇水平显著升高,高密度脂蛋白胆固醇水平显著降低(P<0.05)。与对照组比,肥胖大鼠比目鱼肌毛细血管生成无显著变化,apelin和APJ m RNA表达无显著变化(P>0.05)。与单纯高脂饮食组大鼠相比,10周跑台运动大鼠体重显著降低(P<0.01),血脂改善;此外,比目鱼肌毛细血管密度显著升高,apelin和APJ m RNA表达显著上升(P<0.05),apelin蛋白表达水平显著升高(P<0.05)。结论:跑台运动能显著增加肥胖大鼠比目鱼肌毛细血管生成,其作用可能与运动诱导比目鱼肌apelin表达相关。展开更多
In the present study,a total of 11 compounds were isolated from the aerial parts of Glycyrrhiza uralensis,including two new compounds,glycyuralin Q(1)and glycyuralin R(2),and nine known compounds,including licoripheno...In the present study,a total of 11 compounds were isolated from the aerial parts of Glycyrrhiza uralensis,including two new compounds,glycyuralin Q(1)and glycyuralin R(2),and nine known compounds,including licoriphenone(3),orobol(4),trifoliol(5),7,2′,4′-trihydroxy-5-methoxy-3-arylcoumarin(6),11-hydroxy-9(Z),12(Z)-octadecadienoic acid(7),11-hydroxy-9(E),12(E)-octadecadienoic acid(8),licoricone(9),glycyrin(10),and 2′-hydroxyformononetin(11).Structures of the new compounds were identified by 1 D,2 D NMR and HR-MS data analyses.Compounds 1,2 and 10 showed potent inhibitory activities against PTP1 B,with IC50 values of 1.43,4.71 and 3.79μM,respectively.Compounds 2,4 and 10 inhibitedα-glucosidase with IC50 values of 13.61,11.13 and 17.48μM,respectively.展开更多
文摘目的:观察跑台运动对高脂饮食诱导的肥胖大鼠骨骼肌毛细血管生成及apelin/APJ表达的影响。方法:5周龄健康雄性SD大鼠随机分成对照组和高脂喂养组,分别进行普通饲料和高脂饲料喂养,12周后筛选肥胖大鼠16只,随机分为高脂安静组和高脂运动组。跑台训练持续10周,每周5次,每次60分钟,跑速26米/分钟。测定大鼠体重、体脂重及血脂水平,免疫组化法观察比目鱼肌毛细血管密度和apelin蛋白表达水平,放免法测定比目鱼肌apelin含量,real-time PCR方法测定比目鱼肌apelin和APJ的m RNA表达。结果:与对照组相比,高脂饮食喂养大鼠体重和体脂显著增加(P<0.01),总甘油三酯、总胆固醇和低密度脂蛋白胆固醇水平显著升高,高密度脂蛋白胆固醇水平显著降低(P<0.05)。与对照组比,肥胖大鼠比目鱼肌毛细血管生成无显著变化,apelin和APJ m RNA表达无显著变化(P>0.05)。与单纯高脂饮食组大鼠相比,10周跑台运动大鼠体重显著降低(P<0.01),血脂改善;此外,比目鱼肌毛细血管密度显著升高,apelin和APJ m RNA表达显著上升(P<0.05),apelin蛋白表达水平显著升高(P<0.05)。结论:跑台运动能显著增加肥胖大鼠比目鱼肌毛细血管生成,其作用可能与运动诱导比目鱼肌apelin表达相关。
基金National Natural Science Foundation of China(Grant No.81891010/81891011)National Mega-project for Innovative Drugs(Grant No.2018ZX09711001-008-003)Science&Technology Department of Xinjiang Uygur Autonomous Region(Grant No.2018AB012).
文摘In the present study,a total of 11 compounds were isolated from the aerial parts of Glycyrrhiza uralensis,including two new compounds,glycyuralin Q(1)and glycyuralin R(2),and nine known compounds,including licoriphenone(3),orobol(4),trifoliol(5),7,2′,4′-trihydroxy-5-methoxy-3-arylcoumarin(6),11-hydroxy-9(Z),12(Z)-octadecadienoic acid(7),11-hydroxy-9(E),12(E)-octadecadienoic acid(8),licoricone(9),glycyrin(10),and 2′-hydroxyformononetin(11).Structures of the new compounds were identified by 1 D,2 D NMR and HR-MS data analyses.Compounds 1,2 and 10 showed potent inhibitory activities against PTP1 B,with IC50 values of 1.43,4.71 and 3.79μM,respectively.Compounds 2,4 and 10 inhibitedα-glucosidase with IC50 values of 13.61,11.13 and 17.48μM,respectively.
