目的：通过筛查体检人群幽门螺杆菌（Hp）感染情况，并检测其血清胃蛋白酶原（PG）的含量，探讨 Hp 感染与血清 PG 的关系。方法：选取2013年4月～2014年6月间在江苏省人民医院体检的735名受检者，采用 HG - IRIS13C 红外光谱仪测定 Hp...目的：通过筛查体检人群幽门螺杆菌（Hp）感染情况，并检测其血清胃蛋白酶原（PG）的含量，探讨 Hp 感染与血清 PG 的关系。方法：选取2013年4月～2014年6月间在江苏省人民医院体检的735名受检者，采用 HG - IRIS13C 红外光谱仪测定 Hp 感染情况，时间分辨荧光免疫法（TRFIA）检测血清中 PG I 和 PGⅡ水平。结果：血清 PG I 水平异常组（高于正常组、低于正常组）的 Hp 阳性率明显高于 PG I 水平正常组，差异有统计学意义（P ﹤0.05）；血清 PG II 水平高于正常组的 Hp 阳性率明显高于 PG II 水平正常组，差异有统计学意义（P ﹤0.05）；PG I/ II 比值低于正常组的 Hp 阳性率明显高于正常组，差异有统计学意义（P﹤0.05）。Hp 阳性组的血清 PG I、PG II 水平显著高于阴性组，差异有统计学意义（P ﹤0.05），PG I/Ⅱ比值则显著低于阴性组（P﹤0.05）。Hp 感染与血清 PG I、PG II 水平呈正相关，与血清 PGI/ II 比值呈负相关。结论：血清 PG 水平的变化与 Hp 感染相关。血清 PGI、PGII 水平升高，PG I/ PG II 比值下降提示 HP 感染的可能。展开更多
Eu-chelate were used to construct a two-site sandwich-type assay for pepsinogen Ⅰ （PGI） with time-resolved fluoroimmunoassay （TRFIA） as a detection technique. On the noncompetitive assay, captured monoclonal anti...Eu-chelate were used to construct a two-site sandwich-type assay for pepsinogen Ⅰ （PGI） with time-resolved fluoroimmunoassay （TRFIA） as a detection technique. On the noncompetitive assay, captured monoclonal antibodies （McAbs） coated on wells were directed against a specific antigenic site on the PGI. Another McAbs, called as labeling McAbs, were prepared with the Eu-chelate of N-（p-isothiocyanatobenzyl）-diethylenetriamine-N, N, N, N-tetraacetic acid and directed against a different antigenic site on the PGI. The fluorescence counts of bound Eu^3＋ -McAbs were measured with the auto DELFIA1235 system. The PGI in sera from healthy volunteers were determined by PGI-TRFIA. The within-run and between-run CVs of the PGI-TRFIA were 1.9% and 4.7%, respectively, and the recovery rate was 102.65%. The assay had a detection limit of 0.05 μg· L^-1. The PGI-TRFIA provided a linear response from 3.5 to 328 μg· L^-1. The cross-reacting rate with pepsinogen Ⅱ was negligible. The linear correlation of PGI-TRFIA and radioimmunassay measurements resulted in a correlation coefficient of 0.977. The means of healthy volunteers were 154 ±43 μg·L^-1 for serum PGI. The availability of a highly sensitive, reliable, and convenient method for quantifying PGI will allow investigations into the possible diagnostic value of this analyte in various clinical conditions, including gastric carcinoma, duodenal ulcer, gastritis and severe atrophic gastritis.展开更多
文摘目的：通过筛查体检人群幽门螺杆菌（Hp）感染情况，并检测其血清胃蛋白酶原（PG）的含量，探讨 Hp 感染与血清 PG 的关系。方法：选取2013年4月～2014年6月间在江苏省人民医院体检的735名受检者，采用 HG - IRIS13C 红外光谱仪测定 Hp 感染情况，时间分辨荧光免疫法（TRFIA）检测血清中 PG I 和 PGⅡ水平。结果：血清 PG I 水平异常组（高于正常组、低于正常组）的 Hp 阳性率明显高于 PG I 水平正常组，差异有统计学意义（P ﹤0.05）；血清 PG II 水平高于正常组的 Hp 阳性率明显高于 PG II 水平正常组，差异有统计学意义（P ﹤0.05）；PG I/ II 比值低于正常组的 Hp 阳性率明显高于正常组，差异有统计学意义（P﹤0.05）。Hp 阳性组的血清 PG I、PG II 水平显著高于阴性组，差异有统计学意义（P ﹤0.05），PG I/Ⅱ比值则显著低于阴性组（P﹤0.05）。Hp 感染与血清 PG I、PG II 水平呈正相关，与血清 PGI/ II 比值呈负相关。结论：血清 PG 水平的变化与 Hp 感染相关。血清 PGI、PGII 水平升高，PG I/ PG II 比值下降提示 HP 感染的可能。
文摘Eu-chelate were used to construct a two-site sandwich-type assay for pepsinogen Ⅰ （PGI） with time-resolved fluoroimmunoassay （TRFIA） as a detection technique. On the noncompetitive assay, captured monoclonal antibodies （McAbs） coated on wells were directed against a specific antigenic site on the PGI. Another McAbs, called as labeling McAbs, were prepared with the Eu-chelate of N-（p-isothiocyanatobenzyl）-diethylenetriamine-N, N, N, N-tetraacetic acid and directed against a different antigenic site on the PGI. The fluorescence counts of bound Eu^3＋ -McAbs were measured with the auto DELFIA1235 system. The PGI in sera from healthy volunteers were determined by PGI-TRFIA. The within-run and between-run CVs of the PGI-TRFIA were 1.9% and 4.7%, respectively, and the recovery rate was 102.65%. The assay had a detection limit of 0.05 μg· L^-1. The PGI-TRFIA provided a linear response from 3.5 to 328 μg· L^-1. The cross-reacting rate with pepsinogen Ⅱ was negligible. The linear correlation of PGI-TRFIA and radioimmunassay measurements resulted in a correlation coefficient of 0.977. The means of healthy volunteers were 154 ±43 μg·L^-1 for serum PGI. The availability of a highly sensitive, reliable, and convenient method for quantifying PGI will allow investigations into the possible diagnostic value of this analyte in various clinical conditions, including gastric carcinoma, duodenal ulcer, gastritis and severe atrophic gastritis.
