鱼的雌性特异蛋白(Female specific Protein简称FSP)是雌鱼的特有蛋白一般是从雌鱼血清中经层析、电泳可得到电泳纯雌性特异蛋白。作者采用生化法分离出雌性特异蛋白复合物,并进行免疫双扩散、电泳分析,血清中Ca^(2+)离子及肝脏中葡萄...鱼的雌性特异蛋白(Female specific Protein简称FSP)是雌鱼的特有蛋白一般是从雌鱼血清中经层析、电泳可得到电泳纯雌性特异蛋白。作者采用生化法分离出雌性特异蛋白复合物,并进行免疫双扩散、电泳分析,血清中Ca^(2+)离子及肝脏中葡萄糖六磷酸脱氢酶的测定,以跌卵试验测定其生物活性,发现FSP比对照组有显著高的跌卵作用,将复合物注射给产卵期雌鱼的腹腔及肌肉中,发现鱼血清中Ca^(2+)上升,肝脏中葡萄糖糖磷酸脱氢酶(G-6-PD)活性升高;同步实验显示血清中蛋白质含量及肝脏中RNA含量也显著上升,这些试验结果反映了FSP对鱼体的内部作用机制。展开更多
Trichosanthin (TCS), isolated from the root tuber of Trichosanthes Kirilowii, is atype-Ⅰ eukaryotic ribosome inactivating protein. It has the capacities of inducing mid-termabortion and inhibiting HIV replication. Th...Trichosanthin (TCS), isolated from the root tuber of Trichosanthes Kirilowii, is atype-Ⅰ eukaryotic ribosome inactivating protein. It has the capacities of inducing mid-termabortion and inhibiting HIV replication. The primary structure, gene sequence andthree-dimensional structure of this protein have already been studied.展开更多
文摘鱼的雌性特异蛋白(Female specific Protein简称FSP)是雌鱼的特有蛋白一般是从雌鱼血清中经层析、电泳可得到电泳纯雌性特异蛋白。作者采用生化法分离出雌性特异蛋白复合物,并进行免疫双扩散、电泳分析,血清中Ca^(2+)离子及肝脏中葡萄糖六磷酸脱氢酶的测定,以跌卵试验测定其生物活性,发现FSP比对照组有显著高的跌卵作用,将复合物注射给产卵期雌鱼的腹腔及肌肉中,发现鱼血清中Ca^(2+)上升,肝脏中葡萄糖糖磷酸脱氢酶(G-6-PD)活性升高;同步实验显示血清中蛋白质含量及肝脏中RNA含量也显著上升,这些试验结果反映了FSP对鱼体的内部作用机制。
基金Project supported by the grant of the National "863" High-Tech Project.
文摘Trichosanthin (TCS), isolated from the root tuber of Trichosanthes Kirilowii, is atype-Ⅰ eukaryotic ribosome inactivating protein. It has the capacities of inducing mid-termabortion and inhibiting HIV replication. The primary structure, gene sequence andthree-dimensional structure of this protein have already been studied.
