毛囊球部细胞体外在胶原/壳聚糖多孔支架上形成皮肤样结构的研究

目的 :观察毛囊球部细胞种植到胶原 /壳聚糖多孔支架上皮肤重建情况。方法 :采用细胞培养技术和细胞支架种植技术 ,将培养的毛囊球部细胞种植到胶原 /壳聚糖多孔支架上 ,HE染色观察皮肤样结构形成情况 ,并作免疫组织化学鉴定 ,以证明细胞的来源属性。结果 :毛囊球部细胞种植到胶原 /壳聚糖多孔支架上可见皮肤样结构形成 ,表皮部分细胞分化良好 ,排列复层整齐 ,有角化现象 ,高分子与低分子角蛋白表达阳性。结论 :低传代培养的毛囊球部细胞在体外胶原

Significant reduction of T-cell chemotaxis to MCP-1 in patients with primary and metastatic melanoma

Objective To address whether circulating T cells from the patients with primary and metastatic malignant melanoma show altered chemotaxis to monocyte chemotactic protein 1 (MCP 1) and its relation to tumor infiltrating lymphocyte (TIL) and metastasis Methods Chemotactic response of T cells towards MCP 1 and immuno histochemistry study of TIL and tumor associated macrophages (TAM) were investigated in patients with primary and metastatic melanoma compared to patients with basal cell carcinoma and healthy persons Results T cells from patients with primary and metastatic melanoma showed a significantly decreased chemotactic migration towards MCP 1 and T cells from patients with basal cell carcinoma showed normal chemotactic response Immuno histochemistry study showed that there was no correlation between the number of TIL and the decreased chemotaxis of circulating T cells to MCP 1 in patients with primary melanoma Conclusions Circulating T cells from patients with primary and metastatic melanoma showed a MCP 1 specific decrease in chemotactic migration This may be due to abnormal expression or modulation of MCP 1 receptor expression on these cells