目的:研究光动力法(photodynamic therapy,PDT)处理后,用酸洗法获取C6胶质瘤细胞的抗原肽,制备树突状细胞(dendritic cell,DC)疫苗,诱导细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL)在体外杀伤C6细胞的活性,以及PDT对C6胶质瘤细胞免...目的:研究光动力法(photodynamic therapy,PDT)处理后,用酸洗法获取C6胶质瘤细胞的抗原肽,制备树突状细胞(dendritic cell,DC)疫苗,诱导细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL)在体外杀伤C6细胞的活性,以及PDT对C6胶质瘤细胞免疫原性的直接影响。方法:自大鼠骨髓分离DC前体细胞于体外诱导、扩增获得成熟DC。C6细胞经PDT处理后,对尚贴壁的细胞以酸洗法获取其表面抗原,从上清液中提取全细胞抗原,未作PDT处理的C6细胞以酸洗或冻融法提取抗原。分别以这4种抗原致敏DC,制成DC疫苗接种SD大鼠后,取脾作CTL的体外杀伤活性测定。结果:用PDT处理后酸洗法获得的抗原刺激DC成熟的能力最强,它制备的DC疫苗诱导的CTL的体外杀伤率最高,为(95.5±1.6)%,而光照后上清组为(90.2±2.4)%,酸洗组为(73.3±2.7)%,冻融组为(63.6±4.9)%,PBS对照组为0。结论:PDT能直接增强肿瘤细胞的免疫原性,用酸洗法获取PDT处理后的肿瘤细胞的抗原肽制备DC疫苗是一种有前景的新方法。展开更多
Objective: To examine the changes of osteocalcin and insulin-like growth factor I (IGF-I) during bone lengthening, and to clarify the mechanism of bone healing. Methods: Thirty-two shepherd dogs were divided into five...Objective: To examine the changes of osteocalcin and insulin-like growth factor I (IGF-I) during bone lengthening, and to clarify the mechanism of bone healing. Methods: Thirty-two shepherd dogs were divided into five groups randomly. Their tibiae were lengthened by Ilvzarov’s external fixator at the rate of 1 mm/day. The lengthening area was the experimental side and the opposite side was the control. Samples were obtained on the 2nd, 4th, 6th, 8th, 12th weekend respectively. The samples were defatted, dried, powdered, centrifuged and measured by radioimmunoassay. Results: The osteocalcin concentration increased at the subsequent periods, but it was significantly lower in the experimental side than that of the control side, P<(0.05) and the IGF-I concentration was not significantly lowered. Conclusions: Different noncollagenous bone growth factors may be different at different stage.展开更多
文摘目的:研究光动力法(photodynamic therapy,PDT)处理后,用酸洗法获取C6胶质瘤细胞的抗原肽,制备树突状细胞(dendritic cell,DC)疫苗,诱导细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL)在体外杀伤C6细胞的活性,以及PDT对C6胶质瘤细胞免疫原性的直接影响。方法:自大鼠骨髓分离DC前体细胞于体外诱导、扩增获得成熟DC。C6细胞经PDT处理后,对尚贴壁的细胞以酸洗法获取其表面抗原,从上清液中提取全细胞抗原,未作PDT处理的C6细胞以酸洗或冻融法提取抗原。分别以这4种抗原致敏DC,制成DC疫苗接种SD大鼠后,取脾作CTL的体外杀伤活性测定。结果:用PDT处理后酸洗法获得的抗原刺激DC成熟的能力最强,它制备的DC疫苗诱导的CTL的体外杀伤率最高,为(95.5±1.6)%,而光照后上清组为(90.2±2.4)%,酸洗组为(73.3±2.7)%,冻融组为(63.6±4.9)%,PBS对照组为0。结论:PDT能直接增强肿瘤细胞的免疫原性,用酸洗法获取PDT处理后的肿瘤细胞的抗原肽制备DC疫苗是一种有前景的新方法。
文摘Objective: To examine the changes of osteocalcin and insulin-like growth factor I (IGF-I) during bone lengthening, and to clarify the mechanism of bone healing. Methods: Thirty-two shepherd dogs were divided into five groups randomly. Their tibiae were lengthened by Ilvzarov’s external fixator at the rate of 1 mm/day. The lengthening area was the experimental side and the opposite side was the control. Samples were obtained on the 2nd, 4th, 6th, 8th, 12th weekend respectively. The samples were defatted, dried, powdered, centrifuged and measured by radioimmunoassay. Results: The osteocalcin concentration increased at the subsequent periods, but it was significantly lower in the experimental side than that of the control side, P<(0.05) and the IGF-I concentration was not significantly lowered. Conclusions: Different noncollagenous bone growth factors may be different at different stage.
