Objective To investigate whether adenovirus mediated IL 2 gene modification can improve antitumor immunity of the tumor antigen pulsed dendritic cells(DC) in vivo. Methods DC were generated from bone marrow, then, pul...Objective To investigate whether adenovirus mediated IL 2 gene modification can improve antitumor immunity of the tumor antigen pulsed dendritic cells(DC) in vivo. Methods DC were generated from bone marrow, then, pulsed with FBL 3 erythroleukemia cell lysates and transfected with IL 2 gene simultaneously. IL 2 secretion and T cell stimulating activity of the DC were detected. The number and cell subsets of draining lymph nodes, cytotoxic lymphocyte(CTL) activity ,and the increase of protective effect for the mice s.c vaccinated with DC were observed. Results IL 2 gene modified, antigen pulsed DC could secrete high level of IL 2 in vitro, stimulate proliferation of syngeneic T cells markedly. DC vaccine could increase the number of draining lymph node and induce CTL activity, which directed to FBL 3, more significantly. After the DC vaccination, the survival time of the mice challenged with wild type FBL 3 cells were prolonged. Conclusion Antitumor immune response could be induced more potently by vaccination with IL 2 gene modified, antigen pulsed DC.展开更多
To identify differentially expressed genes from antigen-stimulated human dendritic cells (DC), subtractive cloning was adopted and more than ten novel genes differentially expressed were cloned. One is a deletion muta...To identify differentially expressed genes from antigen-stimulated human dendritic cells (DC), subtractive cloning was adopted and more than ten novel genes differentially expressed were cloned. One is a deletion mutant of heterogeneous nuclear ribonucleoprotein (hnRNP) M4 in which the residues from 159 to 197 of hnRNP M4 have been absent. The deletion mutant was shown to- be co-expressed with hnRNP M4 in cell lines. The mutant was expressed in antigen-stimulated DC but not in normal DC. Northern blot analysis revealed the presence of a major hnRNP M4 deletion mutant mRNA transcript of 2.4 kilobase with the highest levels in peripheral lymphocytes, lung, liver and spleen. It was also expressed in bone marrow-derived stromal cells (BMSC), BMSC treated with several cytokines but not in BMSC treated with TNF-a. The results revealed a new member of hnRNP family and suggested that hnRNP would participate in antigen process and presentation.展开更多
文摘Objective To investigate whether adenovirus mediated IL 2 gene modification can improve antitumor immunity of the tumor antigen pulsed dendritic cells(DC) in vivo. Methods DC were generated from bone marrow, then, pulsed with FBL 3 erythroleukemia cell lysates and transfected with IL 2 gene simultaneously. IL 2 secretion and T cell stimulating activity of the DC were detected. The number and cell subsets of draining lymph nodes, cytotoxic lymphocyte(CTL) activity ,and the increase of protective effect for the mice s.c vaccinated with DC were observed. Results IL 2 gene modified, antigen pulsed DC could secrete high level of IL 2 in vitro, stimulate proliferation of syngeneic T cells markedly. DC vaccine could increase the number of draining lymph node and induce CTL activity, which directed to FBL 3, more significantly. After the DC vaccination, the survival time of the mice challenged with wild type FBL 3 cells were prolonged. Conclusion Antitumor immune response could be induced more potently by vaccination with IL 2 gene modified, antigen pulsed DC.
文摘To identify differentially expressed genes from antigen-stimulated human dendritic cells (DC), subtractive cloning was adopted and more than ten novel genes differentially expressed were cloned. One is a deletion mutant of heterogeneous nuclear ribonucleoprotein (hnRNP) M4 in which the residues from 159 to 197 of hnRNP M4 have been absent. The deletion mutant was shown to- be co-expressed with hnRNP M4 in cell lines. The mutant was expressed in antigen-stimulated DC but not in normal DC. Northern blot analysis revealed the presence of a major hnRNP M4 deletion mutant mRNA transcript of 2.4 kilobase with the highest levels in peripheral lymphocytes, lung, liver and spleen. It was also expressed in bone marrow-derived stromal cells (BMSC), BMSC treated with several cytokines but not in BMSC treated with TNF-a. The results revealed a new member of hnRNP family and suggested that hnRNP would participate in antigen process and presentation.
