川芎坝苓子越夏及其越冬苗的覆盖研究(简报)

在灌县仁羊,川芎(Ligsticum chuanxiong Hort)经历夏、冬二次休眠,夏眠约80天,冬眠30天左右.我们对坝苓子的储藏条件与越冬苗的覆盖等作了调查研究.1

武汉地区32家医院2010-2012年口服降糖药应用分析

目的：分析口服降糖药在武汉地区的应用情况和发展趋势。方法：采用销售金额、用药频度和限定日剂量为指标的分析方法，对武汉地区32家医院2010～2012年口服降糖药相关数据进行统计分析。结果：口服降糖药总体销售金额和用药频度逐年升高，阿卡波糖和格列齐特等位居前列，中药类应用比例下降。结论：武汉地区32家医院的口服降糖药应用基本合理，口服降糖药的市场前景广阔。

PICK1/AMPA在药物成瘾后伏隔核突触可塑性中的研究进展

药物成瘾是由依赖性药物（如吗啡、海洛因、可卡因或酒精等）与大脑奖赏系统相互作用产生的慢性、复发性脑病,主要表现为强迫性用药和对药物的持续性渴求,中脑-边缘多巴胺奖赏系统中的伏隔核（Nucleus accumbens,NAc）和腹侧被盖区是参与药物成瘾最重要的两个核团。

链脲佐菌素和高脂高糖饲料在大鼠2型糖尿病造模中的应用

目的研究2型糖尿病的高效造模方案。方法采用高糖高脂与链脲佐菌素联用制作糖尿病模型,通过大鼠血糖和生活表现来评定方案的优缺性。结果高糖高脂饲料按比例喂养后,加用链脲佐菌素腹腔注射多次,可很好的在大鼠中建立2型糖尿病模型。结论高糖高脂联合链脲佐菌素可成功制作糖尿病模型。

农业水资源高效利用研究探讨

概述了农业水资源的研究现状、概念、理论内涵,强调了实现农业水资源高效利用的条件和途径以及重要现实意义,指出了水资源高效利用研究中存在的不足,以及有待进一步研究的问题,为农业水资源高效利用的进一步的研究提供参考。

凝冻天气对贵州省水利设施的影响分析

根据2008年初贵州雪凝灾害对水利基础设施的损毁客观情况,结合相关调研统计资料,重点对村镇供水设施的损毁特点进行了分析。围绕水利相关行业,从承灾体以及孕灾环境等方面综合分析了此次灾害形成的原因,从灾后恢复重建层面上提出了对策措施,并对今后水利工程规划、设计施工、管理等相关工作的展开提出了建议。研究成果拟对灾区灾后重建和今后加强防范等工作提供参考性意见。

Knockdown of Bmi1 Inhibits Bladder Cancer Cell Growth Both In Vitro and In Vivo by Blocking Cell Cycle at G_1 Phase and Inducing Apoptosis

Bmi1 is a member of the polycomb group family of proteins,and it drives the carcinogenesis of various cancers and governs the self-renewal of multiple types of stem cells. However,its role in the initiation and progression of bladder cancer is not clearly known. The present study aimed to investigate the function of Bmi1 in the development of bladder cancer. Bmi1 expression was detected in human bladder cancer tissues and their adjacent normal tissues（n=10） by immunohistochemistry,q RT-PCR and Western blotting,respectively. Bmi1 small interference RNA（si RNA） was synthesized and transfected into human bladder carcinoma cells（EJ） by lipofectamine 2000. The Bmil expression at m RNA and protein levels was measured in EJ cells transfected with Bmil si RNA（0,80,160 nmol/L） by q RT-PCR and Western blotting,respectively. Cell viability and Ki67 expression（a marker of cell proliferation） were determined in Bmi1 si RNA-transfected cells by CCK-8 assay and q RT-PCR,respectively. Cell cycle of transfected cells was flow-cytometrically determined. Immunofluorescence and Western blotting were used to detect the expression levels of cell cycle-associated proteins cyclin D1 and cyclin E in the cells. Pro-apoptotic proteins Bax and caspase 3 and anti-apoptotic protein Bcl-2 were detected by Western blotting as well. Additionally,xenograft tumor models were established by inoculation of EJ cells（infected with Bmil sh RNA/p LKO.1 lentivirus or not） into nude mice. The tumor volumes were measured every other day for 14 days. The results showed that the Bmil expression was significantly increased in bladder tumor tissues when compared with that in normal tissues（P〈0.05）. Perturbation of Bmi1 expression by using si RNA could significantly inhibit the proliferation of EJ cells（P〈0.05）. Bmi1 si RNA-trasnfected EJ cells were accumulated in G1 phase and the expression levels of cyclin D1 and cyclin E were down-regulated. Bax and caspase-3 expression levels were significantly increased and Bcl-2 levels decreased after Bmi1 knockdown. Tumor volume was conspicuously reduced in mice injected with EJ cells with Bmi1 knockdown. Our findings indicate that Bmi1 is a potential driver oncogene of bladder cancer and it may become a potential treatment target for human bladder cancer.

一棵白菜做出三样美食

“肉中就数猪肉美，菜里从有白菜鲜。”被人们称为“菜中之王”的大白菜，再也不是冬天的“专利品”。夏季吃点白菜，有清热除烦、解渴利尿、瘦身美容的功效。大白菜富含钾、膳食纤维、维生素C等重要的营养素。另外，作为十字花科类蔬菜，大白菜还含有一些异硫氰酸酯等保健元素，对高血压、高血脂、高血糖和便秘都有很好的食疗保健作用。