The effect of ionic hydrodynamic radius in the separation of capillary zone electrophoresis is discussed by using maltooligosaccharide as model compounds. The linear correlation between μep and n-1 (n is the number c...The effect of ionic hydrodynamic radius in the separation of capillary zone electrophoresis is discussed by using maltooligosaccharide as model compounds. The linear correlation between μep and n-1 (n is the number containing the glycosyl units) has been derived and has experimentally been verified with various buffer systems and separation voltages.The results show that hydrodynamic radius is one of the actual factors in HPCZE other than molecular weights. The proposed equation could provide the approach determining glycosyl units of maltooligosaccharide.展开更多
A Gold surface was modified with N acetylcysteine to produce a self assembled monolayer and then a single layer film of rabbit IgG was immmobilized on modified gold surface covalently using carbodiimide hydrochloride(...A Gold surface was modified with N acetylcysteine to produce a self assembled monolayer and then a single layer film of rabbit IgG was immmobilized on modified gold surface covalently using carbodiimide hydrochloride(EDC) and N hydroxysuccinimide(NHS). The competitive method(Ⅰ), the sandwich method(Ⅱ) and the surplus method(Ⅲ) were developed to determine rabbit IgG or goat anti rabbit IgG, respectively. A luminescence analyzer connected with a fluorescence microscope was employed to detect the light intensity of labeled sheep anti rabbit IgG. The results of sensitivity and dynamic range were obtained as 25 mg/L and 25—600 mg/L for method(Ⅰ), 1 0 mg/L and 1—200 mg/L for method(Ⅱ) and 25 mg/L and 25—500 mg/L for method(Ⅲ), respectively.展开更多
文摘The effect of ionic hydrodynamic radius in the separation of capillary zone electrophoresis is discussed by using maltooligosaccharide as model compounds. The linear correlation between μep and n-1 (n is the number containing the glycosyl units) has been derived and has experimentally been verified with various buffer systems and separation voltages.The results show that hydrodynamic radius is one of the actual factors in HPCZE other than molecular weights. The proposed equation could provide the approach determining glycosyl units of maltooligosaccharide.
文摘A Gold surface was modified with N acetylcysteine to produce a self assembled monolayer and then a single layer film of rabbit IgG was immmobilized on modified gold surface covalently using carbodiimide hydrochloride(EDC) and N hydroxysuccinimide(NHS). The competitive method(Ⅰ), the sandwich method(Ⅱ) and the surplus method(Ⅲ) were developed to determine rabbit IgG or goat anti rabbit IgG, respectively. A luminescence analyzer connected with a fluorescence microscope was employed to detect the light intensity of labeled sheep anti rabbit IgG. The results of sensitivity and dynamic range were obtained as 25 mg/L and 25—600 mg/L for method(Ⅰ), 1 0 mg/L and 1—200 mg/L for method(Ⅱ) and 25 mg/L and 25—500 mg/L for method(Ⅲ), respectively.
