Microwave irradiation was firstly attempted to prepare molecularly imprinted polymers(MIPs)monoliths,which were in situ prepared rapidly with methacrylic acid(MAA)as a functional monomer,ethylene glycol dimethacrylate...Microwave irradiation was firstly attempted to prepare molecularly imprinted polymers(MIPs)monoliths,which were in situ prepared rapidly with methacrylic acid(MAA)as a functional monomer,ethylene glycol dimethacrylate(EDMA)as a cross-linking agent,toluene and isooctane as the porogenic solvents and AIBN as an initiator.The baseline separation of isomers of hydroxybenzoic acid was achieved on these monolithic columns with 4-HBA as the template,but not on the blank polymer.Furthermore,some neutral compounds could also be baseline-separated on the imprinted polymer columns in the modes of pressure-driven capillary electrochromatography and low-pressure driven capillary electrochromatography.It was shown that the monoliths were not only used as the molecularly imprinted polymers but also as the reverse phase chromatographic material.展开更多
本实验以干燥厚朴(Magnolia officinalis Rehd. et Wils.)皮为原料,用甲醇的水溶液来进行热浸渍提取法提取有效成分,而后用旋转蒸发仪浓缩,再用正己烷,二氯甲烷,乙酸乙酯,正丁醇依次萃取,分别得到正己烷,二氯甲烷,乙酸乙酯,正丁醇和水...本实验以干燥厚朴(Magnolia officinalis Rehd. et Wils.)皮为原料,用甲醇的水溶液来进行热浸渍提取法提取有效成分,而后用旋转蒸发仪浓缩,再用正己烷,二氯甲烷,乙酸乙酯,正丁醇依次萃取,分别得到正己烷,二氯甲烷,乙酸乙酯,正丁醇和水层五个层。将乙酸乙酯层浓缩后,用硅胶柱层析法进行分离纯化,并依据核磁共振确定其结构。结果表明:分离得到了较纯的起主要药用作用的木质素类化合物1:5,5′-二烯丙基-2,2′-联苯二酚(厚朴酚)和化合物2:5,3′-二烯丙基-2,4′-联苯二酚(和厚朴酚)。展开更多
文摘Microwave irradiation was firstly attempted to prepare molecularly imprinted polymers(MIPs)monoliths,which were in situ prepared rapidly with methacrylic acid(MAA)as a functional monomer,ethylene glycol dimethacrylate(EDMA)as a cross-linking agent,toluene and isooctane as the porogenic solvents and AIBN as an initiator.The baseline separation of isomers of hydroxybenzoic acid was achieved on these monolithic columns with 4-HBA as the template,but not on the blank polymer.Furthermore,some neutral compounds could also be baseline-separated on the imprinted polymer columns in the modes of pressure-driven capillary electrochromatography and low-pressure driven capillary electrochromatography.It was shown that the monoliths were not only used as the molecularly imprinted polymers but also as the reverse phase chromatographic material.
文摘本实验以干燥厚朴(Magnolia officinalis Rehd. et Wils.)皮为原料,用甲醇的水溶液来进行热浸渍提取法提取有效成分,而后用旋转蒸发仪浓缩,再用正己烷,二氯甲烷,乙酸乙酯,正丁醇依次萃取,分别得到正己烷,二氯甲烷,乙酸乙酯,正丁醇和水层五个层。将乙酸乙酯层浓缩后,用硅胶柱层析法进行分离纯化,并依据核磁共振确定其结构。结果表明:分离得到了较纯的起主要药用作用的木质素类化合物1:5,5′-二烯丙基-2,2′-联苯二酚(厚朴酚)和化合物2:5,3′-二烯丙基-2,4′-联苯二酚(和厚朴酚)。
