Using HPLC we purified an antibacterial peptide named CM_2Ph_1 from the hemolymph ofpupae of silkworm (Bombyx mori) immunized with polyI:C. Amino acid composition analysisshowed that CM_2Ph_1 contained 16 kinds of ami...Using HPLC we purified an antibacterial peptide named CM_2Ph_1 from the hemolymph ofpupae of silkworm (Bombyx mori) immunized with polyI:C. Amino acid composition analysisshowed that CM_2Ph_1 contained 16 kinds of amino acids. Its primary structure determined bythe automatic Edman degradation method is GNFFKDLEKMGQRVRDAVISAAPAVDTLAKA-KALGQ. Its C-terminal residue was assumed to be Gln with a blocking α-carboxylamide bycarboxypeptidases analysis. The purified CM_2Ph_1 was found to have inhibition effects invarying degrees on several bacterial strains. At a concentration of 0.35 μmol/L, CM_2Ph_1could inhibit the growth of 50% E. coli D31 strain. Some other antibacterial peptides were compared with CM_2Ph_1 in respect to their struc-tures and functions. Their homology was also discussed in this paper.展开更多
Parasite fly Blepharipa tibialis Chao is one of the pests parasitized in larvae of Antherase pernyi Guer. Organophosphate insecticide dimethoate was found to be very effective against the parasite but not the host. We...Parasite fly Blepharipa tibialis Chao is one of the pests parasitized in larvae of Antherase pernyi Guer. Organophosphate insecticide dimethoate was found to be very effective against the parasite but not the host. We have reported that the selective toxocity of dimethoate may be mainly due to the difference between acetyl-cholinesterase (ACHE) inhibition of nervous system of parasite fly and of A. pernyi. That is to say, the bimolecular reaction constant (Ki) for dimethoate to the展开更多
基金the National Natural Science Foundation of China.
文摘Using HPLC we purified an antibacterial peptide named CM_2Ph_1 from the hemolymph ofpupae of silkworm (Bombyx mori) immunized with polyI:C. Amino acid composition analysisshowed that CM_2Ph_1 contained 16 kinds of amino acids. Its primary structure determined bythe automatic Edman degradation method is GNFFKDLEKMGQRVRDAVISAAPAVDTLAKA-KALGQ. Its C-terminal residue was assumed to be Gln with a blocking α-carboxylamide bycarboxypeptidases analysis. The purified CM_2Ph_1 was found to have inhibition effects invarying degrees on several bacterial strains. At a concentration of 0.35 μmol/L, CM_2Ph_1could inhibit the growth of 50% E. coli D31 strain. Some other antibacterial peptides were compared with CM_2Ph_1 in respect to their struc-tures and functions. Their homology was also discussed in this paper.
文摘Parasite fly Blepharipa tibialis Chao is one of the pests parasitized in larvae of Antherase pernyi Guer. Organophosphate insecticide dimethoate was found to be very effective against the parasite but not the host. We have reported that the selective toxocity of dimethoate may be mainly due to the difference between acetyl-cholinesterase (ACHE) inhibition of nervous system of parasite fly and of A. pernyi. That is to say, the bimolecular reaction constant (Ki) for dimethoate to the
