HPV16 infection is ciosely associated with cervicai carcinomas. A lot or investigation indicate that HPV16 E6/E7 proteins can act as tumor rejection antigens. So an HPV16E6 leukaryoticexpression plasmid pcDNA3/E6 was ...HPV16 infection is ciosely associated with cervicai carcinomas. A lot or investigation indicate that HPV16 E6/E7 proteins can act as tumor rejection antigens. So an HPV16E6 leukaryoticexpression plasmid pcDNA3/E6 was constructed and transfected Into Cos7 cells, which expressed E6protein in vitro. Animal experiments proved that intramuscular immunlzation with plasmid peDNA3/E6 could elicit specific immunoresponse against HPV16 E6 in mice. All these results suggest that E6DNA vaccine can serve as a candidate therapeutic vaccine for HPV16 associated diseases in humans.展开更多
Objective: To determine whether administiation of captopril, an ACE inhibitor, can induce unique gene expression in hearts from spontaneously hypertensive rats (SHR). Methods: Differential screening was used to isolat...Objective: To determine whether administiation of captopril, an ACE inhibitor, can induce unique gene expression in hearts from spontaneously hypertensive rats (SHR). Methods: Differential screening was used to isolate captopril response genes through a human fetal cDNA library, and reverse northern blot technique was applied to analyze gene expression. Results: Administration of captopril in SHR significantly lowered blood pressure, heart rate (HR), and the ratio of heart to body weight was compared with untreated SHR. Three captopril responsive cDNA sequences (expreed sequence tags, ESTs) were identified. One ESTs were highly homologous to human mitochondria gene and other 2 were novel and unknown genes. Conclusion: The benefit of captopril may be mediated, at least in part, by having an effect on the expression of a specific gene involved in cardiac remodeling and function in essential hypertension.展开更多
文摘HPV16 infection is ciosely associated with cervicai carcinomas. A lot or investigation indicate that HPV16 E6/E7 proteins can act as tumor rejection antigens. So an HPV16E6 leukaryoticexpression plasmid pcDNA3/E6 was constructed and transfected Into Cos7 cells, which expressed E6protein in vitro. Animal experiments proved that intramuscular immunlzation with plasmid peDNA3/E6 could elicit specific immunoresponse against HPV16 E6 in mice. All these results suggest that E6DNA vaccine can serve as a candidate therapeutic vaccine for HPV16 associated diseases in humans.
基金National Natural Sciences Foundation of China!No.39725013National Natural Sciences Foundation of China!No.39770341
文摘Objective: To determine whether administiation of captopril, an ACE inhibitor, can induce unique gene expression in hearts from spontaneously hypertensive rats (SHR). Methods: Differential screening was used to isolate captopril response genes through a human fetal cDNA library, and reverse northern blot technique was applied to analyze gene expression. Results: Administration of captopril in SHR significantly lowered blood pressure, heart rate (HR), and the ratio of heart to body weight was compared with untreated SHR. Three captopril responsive cDNA sequences (expreed sequence tags, ESTs) were identified. One ESTs were highly homologous to human mitochondria gene and other 2 were novel and unknown genes. Conclusion: The benefit of captopril may be mediated, at least in part, by having an effect on the expression of a specific gene involved in cardiac remodeling and function in essential hypertension.
