盐田嗜盐单胞杆菌的分离鉴定及其PHB积累的研究

微生物聚合物聚-？-羟基丁酸酯（PHB）具有良好的生物降解性、生物相容性和热塑性,在环保、医药和农业领域具有广阔的应用前景。高PHB积累菌株的获得及其发酵条件的研究具有很好的实用价值。作者从渤海湾日晒盐场盐田卤水中分离出一株杆状细菌,经16S r DNA序列测序比对,确定为盐杆菌属（Halomonas）,命名为Halomonas 100-16-2,其最适生长盐度为50~100,最适生长p H为7~8,属中度嗜盐菌。在酸水解酪蛋白（7.5 g/L）的基础培养基中分别添加0、10、20和30 g/L葡萄糖,研究盐度100条件下不同葡萄糖添加量对Halomonas 100-16-2生长和PHB含量的影响。结果表明,随着葡萄糖浓度的增加,该菌株的生长和PHB积累显著提高。葡萄糖质量浓度为30 g/L时,细胞干质量达8.60 g/L,PHB含量达到5.57 g/L（即达到细胞干质量的65%）。本研究为渤海湾盐田微生物资源的开发和利用提供了有价值的基础数据。

高校贫困生资助体系存在的问题及对策

高校贫困生认定工作是资助工作的首要环节,结合高校资助工作的实际情况,总结高校贫困生资助体系中存在的问题以及相应对策,对于落实国家资助政策,保障贫困生身心健康发展具有重要意义。

关于航海类高职院校学生意识形态调查与思考——以江苏海事职业技术学院为例

为了牢牢把握大学生意识形态工作的领导权,更好地推进马克思主义中国化、时代化、大众化,必须对大学生意识形态工作提出更高的要求。本研究从意识形态、文化渗透、爱国主义教育以及综合素养等几个方面,对航海类高职院校学生的意识形态进行了实证调查,并提出了思考和建议。

Isolation and characterization of halophilic bacteria and archaea from salt ponds in Hangu Saltworks, Tianjin, China

A total of 26 isolates were obtained from solar salt ponds of different salinities(100, 150, 200, and 250) in Hangu Saltworks Co. Ltd., Tianjin, China. Phylogenetic analysis of 16 SrRNA gene sequences indicated that five bacteria genera H alomonas, Salinicoccus, Oceanobacillus, Gracibacillus, and Salimicrobium and one archaea genera H alorubrum were present. The genus H alomonas was predominant with eight strains distributed in a salinity range of 100–200, followed by H alorubrum with six strains in salinity 250. Based on the genus and original sampling salinity, eight bacterial and two archaeal isolates were selected for further morphological, physiological, and biochemical characterization. All of the bacterial strains were moderately halophilic with the optimal salinity for growth being either 50 or 100, while two archaeal strains were extremely halophilic with an optimal growth salinity of 200. Additionally, we put forth strain SM.200-5 as a new candidate S alimicrobium species based on the phylogenic analysis of the 16 SrRNA gene sequence and its biochemical characteristics when compared with known related species.

热力管道保温材料在通行管沟中的应用分析

根据热水管道常用保温材料的技术性能参数和保温效果的影响因素,针对项目热水管道进行了保温材料的选择计算。在选择合理的热水管道保温材料后,对不同工况下热水管道千米温降损失进行计算分析。