杯状病毒科的诺瓦克样病毒和扎幌样病毒两个属主要感染人 ,合称为人类杯状病毒 ( Hu CV) ,Hu CV毒株间具有很大的抗原和遗传学多样性。随着诊断技术的改进 ,对 Hu CV疾病负担有新的估计 ,它是仅次于轮状病毒引起非菌性腹泻的重要病原。...杯状病毒科的诺瓦克样病毒和扎幌样病毒两个属主要感染人 ,合称为人类杯状病毒 ( Hu CV) ,Hu CV毒株间具有很大的抗原和遗传学多样性。随着诊断技术的改进 ,对 Hu CV疾病负担有新的估计 ,它是仅次于轮状病毒引起非菌性腹泻的重要病原。本文简要综述 Hu CV流行病学研究的一些进展。展开更多
The capsid protein encoded by ORF2 of human calicivirus(HuCV) expressed in the baculovirus expression system can self-assembled into virus-like particles(VLPs),which would be important for the development of improving...The capsid protein encoded by ORF2 of human calicivirus(HuCV) expressed in the baculovirus expression system can self-assembled into virus-like particles(VLPs),which would be important for the development of improving diagnostic methods.The RT-PCR procedures routinely used to amplify the RNA polymerase region have usually been inefficient for amplification of ORF2,which would bring obstacle for the development of new diagnostic reagents.Through optimization of RT-PCR conditions,we established a simple and high performance RT-PCR method to amplify ORF2,and by which demonstrated a corresponding relationship between the genotype and the antigenic type.It provides conditions for developing diagnostic reagent which accommodates to diagnosis of HuCVs prevailing in China,and also provides the basis for studying the genetic variance and the pathogenesis of HuCV.展开更多
文摘The capsid protein encoded by ORF2 of human calicivirus(HuCV) expressed in the baculovirus expression system can self-assembled into virus-like particles(VLPs),which would be important for the development of improving diagnostic methods.The RT-PCR procedures routinely used to amplify the RNA polymerase region have usually been inefficient for amplification of ORF2,which would bring obstacle for the development of new diagnostic reagents.Through optimization of RT-PCR conditions,we established a simple and high performance RT-PCR method to amplify ORF2,and by which demonstrated a corresponding relationship between the genotype and the antigenic type.It provides conditions for developing diagnostic reagent which accommodates to diagnosis of HuCVs prevailing in China,and also provides the basis for studying the genetic variance and the pathogenesis of HuCV.
