By means of a series of monoclonal antibodies, gene probes of immanoglobulin aheavy chain (IgH), T cell receptor (TCR) gamma and delta chain as well as chromosome karyotype analysis, the immunophenotype and karyotype ...By means of a series of monoclonal antibodies, gene probes of immanoglobulin aheavy chain (IgH), T cell receptor (TCR) gamma and delta chain as well as chromosome karyotype analysis, the immunophenotype and karyotype were studied in 23 children with acute lymphoblastic leukemia (ALL) diagnosed according to FAB classification criteria and genotype in 21 of them. Among them 16 cases belonged to B lineage ALL, 4 cases to T-ALL, one case to ANLL, and 2 cases to acute undifferential leukemia (AUL). The IgH gene rearrangement was expressed in all of the B lineage ALL, the T-ALL were in germline. The gene rearrangement of TCR gamma and delta chain was expressed in both type of ALL, but the form of rearrangement was different. The quantitative abnormalities of chromosomes were found in 11 of 23 cases, 2 cases had chromosome structure abnormalities. Correlating with clinical manifestations it was found that some relationship between morphology (M), immunology (I), cytogeneties (C), genotype and clinical manifestations in type B-Ⅳ, T-ALL, and A UL exieted, indicating that they had great significance in refining subclassification, prognosis and guidance of treatment in childhood ALLs.展开更多
T cell receptor TCR γ gene rearrangement in a series of acute lymphocytic leukemia (ALL) patients was studied using PCR technique. 18 V-J junctional sequences (designated by N sequence) of TCR γ gene were amplified ...T cell receptor TCR γ gene rearrangement in a series of acute lymphocytic leukemia (ALL) patients was studied using PCR technique. 18 V-J junctional sequences (designated by N sequence) of TCR γ gene were amplified with the Vγ and Jγ primers, using an unsymmetrical PCR and analysed by direct sequencing, demonstrating that in Chinese ALLs, the N sequences of TCR Y gene are indeed clone-specific. Based on the known N sequences, several oligo-nucleotides were sythesized as probes, which were specific for the leukemic clone, and were used to detect the minimal residual disease (MRD) in 4 ALL cases. The sensitivity of this method was 0.1%-0.01%.展开更多
文摘By means of a series of monoclonal antibodies, gene probes of immanoglobulin aheavy chain (IgH), T cell receptor (TCR) gamma and delta chain as well as chromosome karyotype analysis, the immunophenotype and karyotype were studied in 23 children with acute lymphoblastic leukemia (ALL) diagnosed according to FAB classification criteria and genotype in 21 of them. Among them 16 cases belonged to B lineage ALL, 4 cases to T-ALL, one case to ANLL, and 2 cases to acute undifferential leukemia (AUL). The IgH gene rearrangement was expressed in all of the B lineage ALL, the T-ALL were in germline. The gene rearrangement of TCR gamma and delta chain was expressed in both type of ALL, but the form of rearrangement was different. The quantitative abnormalities of chromosomes were found in 11 of 23 cases, 2 cases had chromosome structure abnormalities. Correlating with clinical manifestations it was found that some relationship between morphology (M), immunology (I), cytogeneties (C), genotype and clinical manifestations in type B-Ⅳ, T-ALL, and A UL exieted, indicating that they had great significance in refining subclassification, prognosis and guidance of treatment in childhood ALLs.
基金Project supported by the National Natural Science Foundation of China and Shanghai Natural Science Foundation.
文摘T cell receptor TCR γ gene rearrangement in a series of acute lymphocytic leukemia (ALL) patients was studied using PCR technique. 18 V-J junctional sequences (designated by N sequence) of TCR γ gene were amplified with the Vγ and Jγ primers, using an unsymmetrical PCR and analysed by direct sequencing, demonstrating that in Chinese ALLs, the N sequences of TCR Y gene are indeed clone-specific. Based on the known N sequences, several oligo-nucleotides were sythesized as probes, which were specific for the leukemic clone, and were used to detect the minimal residual disease (MRD) in 4 ALL cases. The sensitivity of this method was 0.1%-0.01%.
