In this paper, by applying fluorescence quenching method and energy transfer technique, the interaction of serum albumin(BSA and HSA) with ketorolac and feprazone was investigated. The binding sites and binding consta...In this paper, by applying fluorescence quenching method and energy transfer technique, the interaction of serum albumin(BSA and HSA) with ketorolac and feprazone was investigated. The binding sites and binding constants were obtained. According to the thermodynamic parameters, the main sorts of binding force can be known. The results show that two drugs and protein have strong interactions. The mechanism of quenching belongs to static quenching and the main sort of binding force is static electricity gravitation.展开更多
为研究多次蒸晒西洋参中皂苷成分变化及其在组织中的空间分布特征,采用超高效液相色谱四极杆飞行时间串联质谱仪(ultra performance liquid chromatography quadrupole time⁃of⁃flight tandem mass spectrometry,UPLC⁃Q⁃TOF⁃MS/MS)对西...为研究多次蒸晒西洋参中皂苷成分变化及其在组织中的空间分布特征,采用超高效液相色谱四极杆飞行时间串联质谱仪(ultra performance liquid chromatography quadrupole time⁃of⁃flight tandem mass spectrometry,UPLC⁃Q⁃TOF⁃MS/MS)对西洋参提取液进行检测和鉴定。采用基质辅助激光解吸电离质谱成像(matrix⁃assisted laser desorption/ionization mass spectrometry imaging,MALDI⁃MSI)对西洋参中原型皂苷及其代谢产物的空间分布和时空变化进行可视化分析。UPLC结果显示从0~9次蒸制西洋参中共鉴定出90种主要人参皂苷,在西洋参的反复蒸晒过程中,极性人参皂苷转化成低极性或非极性人参皂苷,原型皂苷含量减少,稀有皂苷含量增加,主要为人参皂苷20(S/R)⁃Rg_(3)、20(S/R)⁃Rh_(2)、Rk_(1)、Rg_(5)、Rs_(5)、Rs_(4)等。MALDI⁃MSI组织分布结果表明生西洋参中皂苷主要分布于表皮及韧皮部,蒸晒过程导致皂苷成分发生转化,木质部和髓质中的分布增加。该研究为西洋参蒸制引起的生物活性和药理作用的变化提供了基础信息,为扩大西洋参的药用范围提供了参考。展开更多
文摘In this paper, by applying fluorescence quenching method and energy transfer technique, the interaction of serum albumin(BSA and HSA) with ketorolac and feprazone was investigated. The binding sites and binding constants were obtained. According to the thermodynamic parameters, the main sorts of binding force can be known. The results show that two drugs and protein have strong interactions. The mechanism of quenching belongs to static quenching and the main sort of binding force is static electricity gravitation.
文摘为研究多次蒸晒西洋参中皂苷成分变化及其在组织中的空间分布特征,采用超高效液相色谱四极杆飞行时间串联质谱仪(ultra performance liquid chromatography quadrupole time⁃of⁃flight tandem mass spectrometry,UPLC⁃Q⁃TOF⁃MS/MS)对西洋参提取液进行检测和鉴定。采用基质辅助激光解吸电离质谱成像(matrix⁃assisted laser desorption/ionization mass spectrometry imaging,MALDI⁃MSI)对西洋参中原型皂苷及其代谢产物的空间分布和时空变化进行可视化分析。UPLC结果显示从0~9次蒸制西洋参中共鉴定出90种主要人参皂苷,在西洋参的反复蒸晒过程中,极性人参皂苷转化成低极性或非极性人参皂苷,原型皂苷含量减少,稀有皂苷含量增加,主要为人参皂苷20(S/R)⁃Rg_(3)、20(S/R)⁃Rh_(2)、Rk_(1)、Rg_(5)、Rs_(5)、Rs_(4)等。MALDI⁃MSI组织分布结果表明生西洋参中皂苷主要分布于表皮及韧皮部,蒸晒过程导致皂苷成分发生转化,木质部和髓质中的分布增加。该研究为西洋参蒸制引起的生物活性和药理作用的变化提供了基础信息,为扩大西洋参的药用范围提供了参考。