[Objective] The paper was to summarize the advances in studies of toxi- cological effects of benzopyrene on fish immune system. [Method] With the main im- mune factors as the breakthrough points, the paper summarized ...[Objective] The paper was to summarize the advances in studies of toxi- cological effects of benzopyrene on fish immune system. [Method] With the main im- mune factors as the breakthrough points, the paper summarized the toxicity mecha- nism and pathway of benzopyrene on fish immune system, as well as the toxicity mechanism of other environmental organic pollutants on fish immune toxicity. [Remit] Fish immune system was in the special stage of evolution, the study on effect of benzopyrene on fish immune system has great significance. [Conclusion] The paper provided basis for dealing with the immune toxicity caused by organic pollutants and other environmental factors in aquaculture so as to strengthen fish immunity.展开更多
苯并(a)芘(Benzo(a)pyrene,BaP)是一种含有5个苯环的多环芳烃,是环境中广泛存在的一类有机污染物,苯并(a)芘可引起多种形式的DNA损伤。彗星试验,又称为单细胞凝胶电泳(Single Cell Gel Electrophoresis,SCGE),是一项灵敏、快速的在单细...苯并(a)芘(Benzo(a)pyrene,BaP)是一种含有5个苯环的多环芳烃,是环境中广泛存在的一类有机污染物,苯并(a)芘可引起多种形式的DNA损伤。彗星试验,又称为单细胞凝胶电泳(Single Cell Gel Electrophoresis,SCGE),是一项灵敏、快速的在单细胞水平检测DNA链断裂的技术,已经广泛应用于水环境的生物监测中。污染物的毒性监测与评估一直是环境科学研究的热点问题之一。本文通过生态毒理学试验,利用单细胞凝胶电泳技术,研究不同浓度苯并(a)芘暴露对罗非鱼(GIFT Oreochromis niloticus)肝细胞DNA的损伤情况。试验将罗非鱼在0.1、1、10、50μg·L-14个苯并(a)芘(BaP)浓度下分别暴露4、7、14 d,利用彗星试验研究BaP对罗非鱼肝细胞DNA的损伤情况,以尾部DNA含量、尾长、尾矩及Olive尾矩为评价指标,结果表明,BaP会对肝细胞DNA造成不同程度的损伤:在0.1、1、10μg·L-13个浓度组,DNA损伤随着BaP暴露浓度的增加而增加,呈现一定的剂量效应关系,50μg·L-1组DNA损伤有所下降。在时间上,除1μg·L-1剂量组的尾部DNA含量、10μg·L-1组的尾部DNA含量及Olive尾矩外,其他指标有在第7天降低之后又升高的趋势,这意味着肝细胞DNA损伤可在一定程度上反映水体中BaP的污染情况。该实验为进一步探讨苯并(a)芘的致癌机制及环境中苯并(a)芘的监测提供一定的科学依据。展开更多
基金Supported by China Agriculture Research System (CARS-49) National NonprofitInstitute Research Grant of Freshwater Fisheries Research Center,CAFS(2011JBFA16)~~
文摘[Objective] The paper was to summarize the advances in studies of toxi- cological effects of benzopyrene on fish immune system. [Method] With the main im- mune factors as the breakthrough points, the paper summarized the toxicity mecha- nism and pathway of benzopyrene on fish immune system, as well as the toxicity mechanism of other environmental organic pollutants on fish immune toxicity. [Remit] Fish immune system was in the special stage of evolution, the study on effect of benzopyrene on fish immune system has great significance. [Conclusion] The paper provided basis for dealing with the immune toxicity caused by organic pollutants and other environmental factors in aquaculture so as to strengthen fish immunity.
