Objective This study was designed to explore whether Tanshinone ⅡA (TanⅡA) could induce HL-60 and K562 cells differentiation and to assay the telomerase ativities before and after the leukemia cell differentiation i...Objective This study was designed to explore whether Tanshinone ⅡA (TanⅡA) could induce HL-60 and K562 cells differentiation and to assay the telomerase ativities before and after the leukemia cell differentiation induced by TanⅡA and All-trans retinoid acid (ATRA). Methods HL-60 and K562 cells were cultured with TanⅡA at concentration of 0.5μg/ml for 5 days and 6 days respectively. The cell differentiations were observed by morphology and nitroblue tetrazodium (NBT) test. The telomerase activities before and after TanⅡA and ATRA treatment were measured by PCR-TRAP, using ATRA as positive control, 0.01% DMSO as negative control. Results The results showed that TanⅡA inhibited both HL-60 and K562 cell growth. On the 5th day of TanⅡA action, the inhibiting rate of HL-60 cell growth was 79.9%. Also, it induced the cells to differentiate to terminal cells. Acted by TanⅡA for 5 days, the HL-60 cells differentiation rate was 72%, NBT test was positive and showed that the leukemia cells were differentiated. On the 6th day of TanⅡA action, the K562 cells differentiate rate was 76%. The telomerase activities decreased markedly; the inhibiting rates of HL-60 (5 days) and K562 (6 days) were 30.8% and 50.8% respectively. Conclusion These results indicate that TanⅡA induces leukemia cells to differentiate, and with the induction of leukemia cell differentiation, the telomerase activities decrease obviously.展开更多
血液常规检验是最为重要和普及的检验项目之一，但由于各实验室的人员素质的不同，使各实验室之间，甚至同一室的检测结果往往差异较大，如何提高人员的素质．确保血常规检验质量的不断提高。美国病理家学会（College of American Patho...血液常规检验是最为重要和普及的检验项目之一，但由于各实验室的人员素质的不同，使各实验室之间，甚至同一室的检测结果往往差异较大，如何提高人员的素质．确保血常规检验质量的不断提高。美国病理家学会（College of American Pathologists．CAP）实验室认证，帮助我们建立和完善了对血液常规检验人员进行技能培训和能力评估体系，现将主要内容提供给同行参考和借鉴。展开更多
美国病理家学会（college of American pathologists，CAP）实验室认证，主要参照美国临床实验室标准委员会（national committee for clinical laboratory standards，NCCLS）的标准和操作指南制定认证规则，适合临床实验室。在亚洲通...美国病理家学会（college of American pathologists，CAP）实验室认证，主要参照美国临床实验室标准委员会（national committee for clinical laboratory standards，NCCLS）的标准和操作指南制定认证规则，适合临床实验室。在亚洲通过CAP认证的临床实验室，主要在经济较发达的国家（如日本、韩国等）和地区（如中国香港、台湾等）。近几年，随着中国大陆的经济发展，也有少数医院参加CAP认证，我院的临检血液室是通过CAP认证的临床实验室之一。展开更多
文摘Objective This study was designed to explore whether Tanshinone ⅡA (TanⅡA) could induce HL-60 and K562 cells differentiation and to assay the telomerase ativities before and after the leukemia cell differentiation induced by TanⅡA and All-trans retinoid acid (ATRA). Methods HL-60 and K562 cells were cultured with TanⅡA at concentration of 0.5μg/ml for 5 days and 6 days respectively. The cell differentiations were observed by morphology and nitroblue tetrazodium (NBT) test. The telomerase activities before and after TanⅡA and ATRA treatment were measured by PCR-TRAP, using ATRA as positive control, 0.01% DMSO as negative control. Results The results showed that TanⅡA inhibited both HL-60 and K562 cell growth. On the 5th day of TanⅡA action, the inhibiting rate of HL-60 cell growth was 79.9%. Also, it induced the cells to differentiate to terminal cells. Acted by TanⅡA for 5 days, the HL-60 cells differentiation rate was 72%, NBT test was positive and showed that the leukemia cells were differentiated. On the 6th day of TanⅡA action, the K562 cells differentiate rate was 76%. The telomerase activities decreased markedly; the inhibiting rates of HL-60 (5 days) and K562 (6 days) were 30.8% and 50.8% respectively. Conclusion These results indicate that TanⅡA induces leukemia cells to differentiate, and with the induction of leukemia cell differentiation, the telomerase activities decrease obviously.
文摘血液常规检验是最为重要和普及的检验项目之一，但由于各实验室的人员素质的不同，使各实验室之间，甚至同一室的检测结果往往差异较大，如何提高人员的素质．确保血常规检验质量的不断提高。美国病理家学会（College of American Pathologists．CAP）实验室认证，帮助我们建立和完善了对血液常规检验人员进行技能培训和能力评估体系，现将主要内容提供给同行参考和借鉴。
文摘美国病理家学会（college of American pathologists，CAP）实验室认证，主要参照美国临床实验室标准委员会（national committee for clinical laboratory standards，NCCLS）的标准和操作指南制定认证规则，适合临床实验室。在亚洲通过CAP认证的临床实验室，主要在经济较发达的国家（如日本、韩国等）和地区（如中国香港、台湾等）。近几年，随着中国大陆的经济发展，也有少数医院参加CAP认证，我院的临检血液室是通过CAP认证的临床实验室之一。
