目的:探究核受体亚家族4A组成员1(nuclear receptor subfamily 4 group A member 1,Nr4a1)Nr4a1激动剂胞孢子酮B(cytosporone B,Csn-B)对小鼠噪声暴露后听力损失的治疗作用。方法:采用双氧水刺激HEI-OC1毛细胞系的方法构建氧化应激细胞...目的:探究核受体亚家族4A组成员1(nuclear receptor subfamily 4 group A member 1,Nr4a1)Nr4a1激动剂胞孢子酮B(cytosporone B,Csn-B)对小鼠噪声暴露后听力损失的治疗作用。方法:采用双氧水刺激HEI-OC1毛细胞系的方法构建氧化应激细胞模型;通过实时荧光定量PCR(quantitative real-time PCR,q PCR)检测细胞中Nr4a1的mRNA表达水平;分别通过细胞计数试剂盒(cell counting kit-8,CCK8)及流式细胞术的方法检测细胞活力和细胞凋亡水平以评估Csn-B预处理后经双氧水刺激的细胞状态。构建小鼠噪声性听力损失模型,运用qPCR和免疫荧光技术检测噪声暴露后Nr4a1在小鼠耳蜗中的表达;通过检测听性脑干反应(auditory brainstem response,ABR)评估噪声暴露后以及Csn-B连续治疗13 d后小鼠听力情况。结果:双氧水刺激后HEI-OC1毛细胞中Nr4a1表达上升,细胞活力显著下降,凋亡水平显著升高;Csn-B预处理HEI-OC1毛细胞经双氧水刺激,细胞活力显著高于对照组而凋亡水平则显著低于对照组。在体研究结果显示,噪声暴露后小鼠听力显著降低,Nr4a1在小鼠耳蜗中的表达水平显著升高。噪声暴露后经Csn-B治疗小鼠听力得到改善,主要表现为Click-ABR以及Tone Burst-ABR(4000、8000Hz处)阈值下降。结论:Nr4a1激动剂Csn-B增强内耳毛细胞对氧化应激损伤的抵御能力,部分改善噪声暴露后的小鼠听力。展开更多
目的探讨耳内镜下儿童局限性中耳胆脂瘤分期手术的可行性。方法对5例儿童单侧中耳胆脂瘤患者在耳内镜下进行一期鼓膜切除、取出听骨链及清理胆脂瘤病变,3~6个月后于耳内镜下行鼓室探查、听骨链重建及鼓膜修补,分析耳内镜下分期手术的效...目的探讨耳内镜下儿童局限性中耳胆脂瘤分期手术的可行性。方法对5例儿童单侧中耳胆脂瘤患者在耳内镜下进行一期鼓膜切除、取出听骨链及清理胆脂瘤病变,3~6个月后于耳内镜下行鼓室探查、听骨链重建及鼓膜修补,分析耳内镜下分期手术的效果及体会。结果二期术后随访半年,5例患儿术耳平均气导听阈为28.32±1.17 dB HL,平均气骨导差为15.66±3.21 dB,与术前(42±3.79 dB HL)比较听力明显提高(P<0.01),复查鼓膜移植物在位、愈合良好,无内陷或穿孔。结论耳内镜下分期手术治疗儿童局限性中耳胆脂瘤能明显减少手术对中耳及乳突结构和功能的破坏,术后患者听力提高及鼓膜愈合效果好。展开更多
Age-related hearing loss（AHL） is one of the most common sensory disorders among elderly persons. The inwardly rectifying potassium channel 5.1（Kir5.1） plays a vital role in regulating cochlear K~＋ circulation whi...Age-related hearing loss（AHL） is one of the most common sensory disorders among elderly persons. The inwardly rectifying potassium channel 5.1（Kir5.1） plays a vital role in regulating cochlear K~＋ circulation which is necessary for normal hearing. The distribution of Kir5.1 in C57BL/6J mice cochleae, and the relationship between the expression of Kir5.1 and the etiology of AHL were investigated. Forty C57BL/6J mice were randomly divided into four groups at 4, 12, 24 and 52 weeks of age respectively. The location of Kir5.1 was detected by immunofluorescence technique. The m RNA and protein expression of Kir5.1 was evaluated in mice cochleae using real-time polymerase-chain reactions（RT-PCR） and Western blotting respectively. Kir5.1 was detected in the type Ⅱ and Ⅳfibrocytes of the spiral ligament in the cochlear lateral wall of C57BL/6J mice. The expression levels of Kir5.1 m RNA and protein in the cochleae of aging C57BL/6J mice were down-regulated. It was suggested that the age-related decreased expression of Kir5.1 in the lateral wall of C57BL/6J mice was associated with hearing loss. Our results indicated that Kir5.1 may play an important role in the pathogenesis of AHL.展开更多
文摘目的探讨耳内镜下儿童局限性中耳胆脂瘤分期手术的可行性。方法对5例儿童单侧中耳胆脂瘤患者在耳内镜下进行一期鼓膜切除、取出听骨链及清理胆脂瘤病变,3~6个月后于耳内镜下行鼓室探查、听骨链重建及鼓膜修补,分析耳内镜下分期手术的效果及体会。结果二期术后随访半年,5例患儿术耳平均气导听阈为28.32±1.17 dB HL,平均气骨导差为15.66±3.21 dB,与术前(42±3.79 dB HL)比较听力明显提高(P<0.01),复查鼓膜移植物在位、愈合良好,无内陷或穿孔。结论耳内镜下分期手术治疗儿童局限性中耳胆脂瘤能明显减少手术对中耳及乳突结构和功能的破坏,术后患者听力提高及鼓膜愈合效果好。
基金supported by the National Natural Science Foundation of China(Nos.81271078,81300827,and 81500791)
文摘Age-related hearing loss（AHL） is one of the most common sensory disorders among elderly persons. The inwardly rectifying potassium channel 5.1（Kir5.1） plays a vital role in regulating cochlear K~＋ circulation which is necessary for normal hearing. The distribution of Kir5.1 in C57BL/6J mice cochleae, and the relationship between the expression of Kir5.1 and the etiology of AHL were investigated. Forty C57BL/6J mice were randomly divided into four groups at 4, 12, 24 and 52 weeks of age respectively. The location of Kir5.1 was detected by immunofluorescence technique. The m RNA and protein expression of Kir5.1 was evaluated in mice cochleae using real-time polymerase-chain reactions（RT-PCR） and Western blotting respectively. Kir5.1 was detected in the type Ⅱ and Ⅳfibrocytes of the spiral ligament in the cochlear lateral wall of C57BL/6J mice. The expression levels of Kir5.1 m RNA and protein in the cochleae of aging C57BL/6J mice were down-regulated. It was suggested that the age-related decreased expression of Kir5.1 in the lateral wall of C57BL/6J mice was associated with hearing loss. Our results indicated that Kir5.1 may play an important role in the pathogenesis of AHL.
