草菇液体菌种培养条件的对比试验

为了筛选合适的草菇液体菌种培养条件,本实验设计三个配方进行草菇液体菌种摇床培养对比试验,筛选出适宜的培养基配方,即马铃薯50 g,葡萄糖20 g,蛋白胨1.5 g,KH2PO4 1 g,MgSO4 0.5 g,维生素B1 10 mg,水1000 mL,pH自然。在此基础上设计摇床转速、维生素B1添加浓度、培养基装瓶量、摇前静置时间等四个因子,进行草菇液体菌种摇床培养的比较试验。结果表明,草菇液体菌种培养最适合的摇床转速是130 r/min;添加的维生素B1的最佳浓度为15 mg/L;规格为250 mL的锥形瓶,培养基的最适装瓶量为80 mL;培养基静置36 h再进行摇床培养效果最好。In order to screen suitable culture conditions of liquid strains for Volvariella volvacea, this experiment designed three formulas for comparative shaking table cultivation of V. volvacea liquid strains. The suitable medium formula was selected, namely 50 g of potato, 20 g of glucose, 1.5 g of peptone, 1 g of KH2PO4, 0.5 g of MgSO4, 10 mg of Vitamin B1, 1000 mL of water, and natural pH. On this basis, four factors were designed, including shaking table speed, vitamin B1 addition concentration, bottling volume of culture medium, and settling time before shaking, to conduct comparative experiments on V. volvacea liquid strain shaking table cultivation. The results showed that the most suitable shaking table speed for the cultivation of V. volvacea liquid strains was 130 r/min;the optimal concentration of added vitamin B1 was 15 mg/L;the optimal filling volume for a 250 mL conical flask and culture medium was 80 mL;the best effect was to let the culture medium stand for 36 hours before shaking it on a shaker.

矿质元素对茶树菇液体菌种菌丝生长的影响

采用摇床培养的方法,以菌丝球的生物量为评价指标,通过单因素试验和正交试验,说明Zn、Fe、Mn、Cu 四种矿质元素对茶树菇液体菌丝生长的影响。结果表明:Zn、Fe、Mn、Cu四种矿质元素对茶树菇液体菌种菌丝生长具有促进作用,其中Zn对茶树菇菌丝生长的影响最明显,其次为Fe、Mn、Cu。这四种矿质元素可以通过单独添加发挥作用,也可以混合添加共同作用,混合添加比单独添加效果更好,促进茶树菇菌丝生长的最优组合是0.25 g/L ZnSO4·7H2O,0.30 g/L FeSO4·7H2O,0.20 g/L MnSO4·H2O,0.15 g/L CuSO4·5H2O。Using the shaking table cultivation method and using the biomass of mycelium balls as the evaluation index, single factor experiments and orthogonal experiments were conducted to illustrate the effects of four mineral elements, Zn, Fe, Mn, and Cu, on the liquid mycelial growth of Agrocybe aegerita. The results showed that four mineral elements, Zn, Fe, Mn, and Cu, had a promoting effect on the mycelial growth of liquid Agrocybe aegerita strains. Among them, Zn had the most significant effect on the mycelial growth of Agrocybe aegerita, followed by Fe, Mn, and Cu. These four mineral elements can exert their effects by adding them alone or by mixing them together. The mixed addition has a better effect than adding them alone. The optimal combination to promote the mycelial growth of Agrocybe aegerita is 0.25 g/L ZnSO4·7H2O, 0.30 g/L FeSO4·7H2O, 0.20 g/L MnSO4·H2O, and 0.15 g/L CuSO4·5H2O.

利用木薯秆栽培赤芝的试验

为了充分利用广西丰富的农业资源,提高农业综合效益,以木薯秆为主要原料,以桑枝杆作为对照,设置木薯秆的占比分别为70%、55%、40%等三组,每组再设置三个处理,共9个处理,进行赤芝栽培试验,探讨木薯秆栽培赤芝的可行性和高产优质的栽培配方,结果表明:利用木薯秆作为主要原料栽培赤芝是可行的,单独以木薯秆作为碳源,或在木薯秆中添加适当的桑杆、甘蔗渣,均能获得较好的栽培效果,其中,在木薯秆中添加30%的甘蔗渣栽培效果最好。In order to fully utilize the abundant agricultural resources in Guangxi and improve the comprehensive agricultural benefits, cassava stalks were used as the main raw material, and mulberry branches were used as the control. Three groups of cassava stalks were set with proportions of 70%, 55%, and 40%, respectively, and three treatments were set for each group, for a total of nine treatments. The cultivation experiment of Ganoderma lucidum was conducted to explore the feasibility of cultivating G. lucidum with cassava stalks and the high-yield and high-quality cultivation formula. The results showed that using cassava stalks as the main raw material to cultivate G. lucidum was feasible. Using cassava stalks as a carbon source alone or adding appropriate mulberry stalks and sugarcane bagasse to cassava stalks could achieve good cultivation effects. Among them, adding 30% sugarcane bagasse to cassava stalks had the best cultivation effect.

杏鲍菇母种培养基配方优化的试验

为了筛选杏鲍菇母种培养基的优化配方,采用单因素试验和正交试验方法,通过设置不同碳源添加量、氮源添加量和无机盐添加量,以菌丝生长速度、菌丝浓度、气生菌丝量和边缘整齐度为主要评价指标,筛选出杏鲍菇母种培养基的最佳配方。结果表明,在培养温度为25˚C,葡萄糖20 g/L,琼脂20 g/L,接种菌块直径为6 mm时,单因素试验筛选得到的玉米芯最适添加量为450 g/L,麦麸最适添加量为60 g/L,无机盐的最适添加量为3.8 g/L;正交试验筛选出的杏鲍菇母种培养基最佳配方为玉米芯450 g/L、麦麸50 g/L、硫酸镁4.2 g/L。利用玉米芯等农业生产废弃物进行杏鲍菇母种栽培,不仅能够节约资源能源,减少环境污染,还能够有效降低成本,变废为宝,提高经济效益。

灵芝的板栗苞壳菌糠栽培平菇试验

将板栗苞壳栽培灵芝的菌糠以50%、30%、10%的比例添加到培养基中,以桑枝屑为对照进行平菇栽培试验。结果表明:灵芝菌糠对平菇菌丝生长有明显促进作用,平菇的产量、生物学效率及商品性状与对照组相当,利用板栗苞壳栽培灵芝的菌糠种植平菇,不仅可以充分挖掘板栗苞壳在食用菌栽培方面的利用价值,还可以节约资源,保护环境。