Four cell lines originated from embryo,caudal fin,anal fin and snout of Colossoma brachypomum were established,and subcultured for 70,47,45 and 40 passages to the present.The results of the experiment indicated that t...Four cell lines originated from embryo,caudal fin,anal fin and snout of Colossoma brachypomum were established,and subcultured for 70,47,45 and 40 passages to the present.The results of the experiment indicated that the four cell lines were fibroblast\|like diploid cells,and the model number of their chromosome is 54.These cell lines could grow under temperature of 20-35℃,and its optimum temperature was 27-30℃.The cells grew slowly at 20℃,stopped growth at 15℃,and died below 10℃.This indicated that the growth temperature of the these cell lines was coincident with that of fish.In addition,the attachment time,doubling time,mitotic index,passage survival rate and cell cycle were determined and compared.展开更多
本文介绍了阶段特异性胚胎抗原(stage specific embryonic antigen,SSEA)的发现、化学结构和在胚胎、成熟组织中的分布规律;SSEA通过参与细胞间的信息传递、细胞识别与粘附等过程在动物胚胎发育、组织分化、基因调控及肿瘤的发生、转移...本文介绍了阶段特异性胚胎抗原(stage specific embryonic antigen,SSEA)的发现、化学结构和在胚胎、成熟组织中的分布规律;SSEA通过参与细胞间的信息传递、细胞识别与粘附等过程在动物胚胎发育、组织分化、基因调控及肿瘤的发生、转移等方面发挥重要作用;SSEA作为细胞表面标志,在肿瘤患者确诊、预后监测及胚胎干细胞的分离纯化、鉴定等研究领域的重要应用价值;探讨了SSEA-1合成的关键酶——岩藻糖基转移酶基因表达与调控的研究进展。展开更多
文摘Four cell lines originated from embryo,caudal fin,anal fin and snout of Colossoma brachypomum were established,and subcultured for 70,47,45 and 40 passages to the present.The results of the experiment indicated that the four cell lines were fibroblast\|like diploid cells,and the model number of their chromosome is 54.These cell lines could grow under temperature of 20-35℃,and its optimum temperature was 27-30℃.The cells grew slowly at 20℃,stopped growth at 15℃,and died below 10℃.This indicated that the growth temperature of the these cell lines was coincident with that of fish.In addition,the attachment time,doubling time,mitotic index,passage survival rate and cell cycle were determined and compared.
文摘本文介绍了阶段特异性胚胎抗原(stage specific embryonic antigen,SSEA)的发现、化学结构和在胚胎、成熟组织中的分布规律;SSEA通过参与细胞间的信息传递、细胞识别与粘附等过程在动物胚胎发育、组织分化、基因调控及肿瘤的发生、转移等方面发挥重要作用;SSEA作为细胞表面标志,在肿瘤患者确诊、预后监测及胚胎干细胞的分离纯化、鉴定等研究领域的重要应用价值;探讨了SSEA-1合成的关键酶——岩藻糖基转移酶基因表达与调控的研究进展。
