骨髓增生异常综合征(myelodysplastic syndrome,MDS)是一组起源于造血干细胞,以髓系细胞发育异常、无效造血、难治性血细胞减少、高风险向急性髓系白血病转化为特点的异质性髓系克隆性疾病[1]。弥漫大B细胞淋巴瘤(diffuse large B cell ...骨髓增生异常综合征(myelodysplastic syndrome,MDS)是一组起源于造血干细胞,以髓系细胞发育异常、无效造血、难治性血细胞减少、高风险向急性髓系白血病转化为特点的异质性髓系克隆性疾病[1]。弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)起源于淋巴结和淋巴组织,是最常见的非霍奇金淋巴瘤,占比30%~40%[2],在临床表现、预后等方面具有较大异质性。展开更多
Objective: To explore the effects of Danshen Injection (丹参注射液) on inhibition proliferation, inducing apoptosis and its possible mechanisms on human erythroid leukemic (HEL) cells. Methods: The commercial Ch...Objective: To explore the effects of Danshen Injection (丹参注射液) on inhibition proliferation, inducing apoptosis and its possible mechanisms on human erythroid leukemic (HEL) cells. Methods: The commercial Chinese patent medicine of Danshen Injection was extracted and isolated from Chinese herb of Salvia miltiorrhiza bung. The inhibition effects of proliferation were assayed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (M'l-r) method in HEL cells treated by Danshen Injection at various concentrations for 48 h. The cellular apoptosis was observed in morphology, analyzed by flow cytometry with annexin V and propidium iodide (PI) staining, and examined by DNA degradation ladder on agarose gel electrophoresis. Meanwhile, the expression levels of mutant Janus kinasez (JAK2) gene and phosphorylation-JAK2 (P-JAK2) protein were detected by allele specific-polymerase chain reaction and Western blot. ]Results: The proliferation of HEL cells was effectively inhibited by Danshen Injection in a dose-dependent manner, with suppression rates from 19.46 ± 2.31% to 50.20 ± 5.21%. Typical apoptosis cells was observed in Danshen Injection treated HEL cells, the rates of annexin V positive cells increased obviously in a dose-dependent manner, as well as the DNA degradation ladder of apoptosis revealed on gel electrophoresis. The expression levels of mutant JAK2 gene and P-JAK2 protein reduced gradually with increasing dosage of Danshen injection. Conclusion: Danshen Injection could not only significantly inhibit the proliferation, but also induce apoptosis in HEL cells; down-regulation of the mutant JAK2 gene and P-JAK2 protein expressions are probably one of its molecular mechanisms.展开更多
文摘骨髓增生异常综合征(myelodysplastic syndrome,MDS)是一组起源于造血干细胞,以髓系细胞发育异常、无效造血、难治性血细胞减少、高风险向急性髓系白血病转化为特点的异质性髓系克隆性疾病[1]。弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)起源于淋巴结和淋巴组织,是最常见的非霍奇金淋巴瘤,占比30%~40%[2],在临床表现、预后等方面具有较大异质性。
基金Supported by Zhejiang Province Administration of Traditional Chinese Medicine(No.2010ZA120)Zhejiang Provincial Natural Science Foundation of China(No.Y207632)
文摘Objective: To explore the effects of Danshen Injection (丹参注射液) on inhibition proliferation, inducing apoptosis and its possible mechanisms on human erythroid leukemic (HEL) cells. Methods: The commercial Chinese patent medicine of Danshen Injection was extracted and isolated from Chinese herb of Salvia miltiorrhiza bung. The inhibition effects of proliferation were assayed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (M'l-r) method in HEL cells treated by Danshen Injection at various concentrations for 48 h. The cellular apoptosis was observed in morphology, analyzed by flow cytometry with annexin V and propidium iodide (PI) staining, and examined by DNA degradation ladder on agarose gel electrophoresis. Meanwhile, the expression levels of mutant Janus kinasez (JAK2) gene and phosphorylation-JAK2 (P-JAK2) protein were detected by allele specific-polymerase chain reaction and Western blot. ]Results: The proliferation of HEL cells was effectively inhibited by Danshen Injection in a dose-dependent manner, with suppression rates from 19.46 ± 2.31% to 50.20 ± 5.21%. Typical apoptosis cells was observed in Danshen Injection treated HEL cells, the rates of annexin V positive cells increased obviously in a dose-dependent manner, as well as the DNA degradation ladder of apoptosis revealed on gel electrophoresis. The expression levels of mutant JAK2 gene and P-JAK2 protein reduced gradually with increasing dosage of Danshen injection. Conclusion: Danshen Injection could not only significantly inhibit the proliferation, but also induce apoptosis in HEL cells; down-regulation of the mutant JAK2 gene and P-JAK2 protein expressions are probably one of its molecular mechanisms.