采用随机方法分析了蕴含轴向流动流体的BTA(boring and trepanning association)深孔镗杆在随机力下的横向随机动力行为。建模时考察了流固耦合镗杆承受的弯曲、拉伸和扭转变形;经Galerkin Method离散化处理,分析了BTA镗杆在有、无随机...采用随机方法分析了蕴含轴向流动流体的BTA(boring and trepanning association)深孔镗杆在随机力下的横向随机动力行为。建模时考察了流固耦合镗杆承受的弯曲、拉伸和扭转变形;经Galerkin Method离散化处理,分析了BTA镗杆在有、无随机激励两种情况的特征值和特征频率对振动特性的影响;利用响应方差最大值和谱密度解析了BTA镗杆横向振动的临界转速与临界失稳频率;明确了镗杆随转速、刚度、初始轴向总力和剪切模量等参数变化对系统振动特性的影响机制:镗杆转速变化对系统稳定性不再具有单调性,随BTA镗杆转速持续增加,系统可历经两次转速的临界失稳,相继出现二次失稳和二次稳定;增加系统等效刚度和等效剪切模量会促进工作过程的稳定,改变轴向力对工作过程稳定的影响不明显;并以随机振动物理试验信号的功率谱分析,验证了理论仿真结果与试验结果的一致性。该研究在一定程度上揭示了BTA深孔工艺系统运动状态的复杂性,这种研究模式为进一步分析在复杂状态下的运动演化提供了更多的可能。研究结论为更好地理解BTA深孔镗杆工作时的随机动力行为提供了依据,也为BTA深孔工艺过程的振动控制和参数优化奠定了理论基础。展开更多
To construct the bi-valent genetic engineering vaccine against pseudorabies virus（PRV）and porcine reproductive and respiratory syndrome virus（PRRSV）,the modified PRRSV ORF5 gene（ORF5M） and the VP22 gene of bovin...To construct the bi-valent genetic engineering vaccine against pseudorabies virus（PRV）and porcine reproductive and respiratory syndrome virus（PRRSV）,the modified PRRSV ORF5 gene（ORF5M） and the VP22 gene of bovine herpesvirus 1（BHV-1）,which encodes VP22 protein and has been demonstrated to exhibit the unusual protein transduction property,were inserted into a PRV universal transfer vector pIECMV by turns.A recombinant virus transfer vector pIECMV-VP22ORF5M possessing VP22-ORF5M fusion gene was generated.The recombinant virus transfer vector pIECMV-VP22ORF5M co-transfected the IBRS-2 cells with PRV TK-/gE-/LacZ+ genomic DNA digested by EcoRⅠusing liposome method.Based on homologous recombination,the recombinant virus was generated and then purified by the plaque assay and PCR amplification.After three rounds of plaque purification,the recombinant virus was further confirmed by PCR,Southern blot and Western blot.A recombinant PRV（rPRV）TK-/gE-/VP22GP5+ expressing VP22-GP5 fusion protein was constructed.The results of TCID50 tests showed that the insertion of the foreign genes had no influence on the propagation of rPRV in IBRS-2 or PK-15 cells.The construction of rPRV TK-/gE-/VP22GP5+ provides a basis for further study of bi-valent genetic engineering vaccines against PRRSV and PRV,and that this strategy may also be useful to develop more efficient genetic engineering vaccines against other pathogens.展开更多
随着全球化的发展,中国传统集体主义价值观日渐受到西方个人主义价值观的冲击。本文基于Triandis的个人主义和集体主义理论,采用Triandis集体主义量表和Heneman多维度薪酬满意度量表对中国国有科研机构员工进行问卷调查,运用spss15.0 fo...随着全球化的发展,中国传统集体主义价值观日渐受到西方个人主义价值观的冲击。本文基于Triandis的个人主义和集体主义理论,采用Triandis集体主义量表和Heneman多维度薪酬满意度量表对中国国有科研机构员工进行问卷调查,运用spss15.0 for windows计算文化价值取向各维度与薪酬满意度各维度之间的相关系数,从文化价值取向的视角探讨不同价值取向的科研人员的薪酬满意度差异。展开更多
文摘采用随机方法分析了蕴含轴向流动流体的BTA(boring and trepanning association)深孔镗杆在随机力下的横向随机动力行为。建模时考察了流固耦合镗杆承受的弯曲、拉伸和扭转变形;经Galerkin Method离散化处理,分析了BTA镗杆在有、无随机激励两种情况的特征值和特征频率对振动特性的影响;利用响应方差最大值和谱密度解析了BTA镗杆横向振动的临界转速与临界失稳频率;明确了镗杆随转速、刚度、初始轴向总力和剪切模量等参数变化对系统振动特性的影响机制:镗杆转速变化对系统稳定性不再具有单调性,随BTA镗杆转速持续增加,系统可历经两次转速的临界失稳,相继出现二次失稳和二次稳定;增加系统等效刚度和等效剪切模量会促进工作过程的稳定,改变轴向力对工作过程稳定的影响不明显;并以随机振动物理试验信号的功率谱分析,验证了理论仿真结果与试验结果的一致性。该研究在一定程度上揭示了BTA深孔工艺系统运动状态的复杂性,这种研究模式为进一步分析在复杂状态下的运动演化提供了更多的可能。研究结论为更好地理解BTA深孔镗杆工作时的随机动力行为提供了依据,也为BTA深孔工艺过程的振动控制和参数优化奠定了理论基础。
文摘To construct the bi-valent genetic engineering vaccine against pseudorabies virus（PRV）and porcine reproductive and respiratory syndrome virus（PRRSV）,the modified PRRSV ORF5 gene（ORF5M） and the VP22 gene of bovine herpesvirus 1（BHV-1）,which encodes VP22 protein and has been demonstrated to exhibit the unusual protein transduction property,were inserted into a PRV universal transfer vector pIECMV by turns.A recombinant virus transfer vector pIECMV-VP22ORF5M possessing VP22-ORF5M fusion gene was generated.The recombinant virus transfer vector pIECMV-VP22ORF5M co-transfected the IBRS-2 cells with PRV TK-/gE-/LacZ+ genomic DNA digested by EcoRⅠusing liposome method.Based on homologous recombination,the recombinant virus was generated and then purified by the plaque assay and PCR amplification.After three rounds of plaque purification,the recombinant virus was further confirmed by PCR,Southern blot and Western blot.A recombinant PRV（rPRV）TK-/gE-/VP22GP5+ expressing VP22-GP5 fusion protein was constructed.The results of TCID50 tests showed that the insertion of the foreign genes had no influence on the propagation of rPRV in IBRS-2 or PK-15 cells.The construction of rPRV TK-/gE-/VP22GP5+ provides a basis for further study of bi-valent genetic engineering vaccines against PRRSV and PRV,and that this strategy may also be useful to develop more efficient genetic engineering vaccines against other pathogens.
文摘随着全球化的发展,中国传统集体主义价值观日渐受到西方个人主义价值观的冲击。本文基于Triandis的个人主义和集体主义理论,采用Triandis集体主义量表和Heneman多维度薪酬满意度量表对中国国有科研机构员工进行问卷调查,运用spss15.0 for windows计算文化价值取向各维度与薪酬满意度各维度之间的相关系数,从文化价值取向的视角探讨不同价值取向的科研人员的薪酬满意度差异。
