Objective To study on the CDKN2/P16gene in prim ary osteosarcoma.Method By using molecular biological methods that inclued genome DNA extraction from p araffined tissue and PCR-SSCP analy sis technique,we studied alte...Objective To study on the CDKN2/P16gene in prim ary osteosarcoma.Method By using molecular biological methods that inclued genome DNA extraction from p araffined tissue and PCR-SSCP analy sis technique,we studied alternations of CDKN2/P16gene in 25primary osteosarcomas.Results(1)The deletions frequency in differen tiation degree of osteosarcomas was①bone brood cell,16.7%;②cartilage brood cell,12.5%;③Fiber brood cell:20%,(P >0.05).(2)The deletion frequency in male patie nts was 17.6%,female pa-tients 12.5%,(P >0.05).(3)In early metastatic osteosarcomas t he deletion rate was 33.3%,which was significantly higher than that of th e control group with the rate of 10.5%(P <0.05).(4)The deletion rate was 16%and the muta tions were not found.Conclusion(1)The deletion rate was 16%and the mutatio ns were not found.This suggests that the deletions of CDKN2/P16gene were closely related to the genesis of primary osteosarcoma and that the main type of the alternation of CDKN2/P16gene was deletion.(2)In early metastatic osteosaarcomas the deletion rate was 33.3%,which was significantly higher than that of the c ontrol group with the rate of 10.5%.T his indicates to great extend that the deletions of CDKN2/P16gene were closely related to the metastat ic ability.(3)The deletions frequency had no signi ficant relationship with differentiation degree of oste osarcomas,so was with the sex of the p atient.展开更多
文摘Objective To study on the CDKN2/P16gene in prim ary osteosarcoma.Method By using molecular biological methods that inclued genome DNA extraction from p araffined tissue and PCR-SSCP analy sis technique,we studied alternations of CDKN2/P16gene in 25primary osteosarcomas.Results(1)The deletions frequency in differen tiation degree of osteosarcomas was①bone brood cell,16.7%;②cartilage brood cell,12.5%;③Fiber brood cell:20%,(P >0.05).(2)The deletion frequency in male patie nts was 17.6%,female pa-tients 12.5%,(P >0.05).(3)In early metastatic osteosarcomas t he deletion rate was 33.3%,which was significantly higher than that of th e control group with the rate of 10.5%(P <0.05).(4)The deletion rate was 16%and the muta tions were not found.Conclusion(1)The deletion rate was 16%and the mutatio ns were not found.This suggests that the deletions of CDKN2/P16gene were closely related to the genesis of primary osteosarcoma and that the main type of the alternation of CDKN2/P16gene was deletion.(2)In early metastatic osteosaarcomas the deletion rate was 33.3%,which was significantly higher than that of the c ontrol group with the rate of 10.5%.T his indicates to great extend that the deletions of CDKN2/P16gene were closely related to the metastat ic ability.(3)The deletions frequency had no signi ficant relationship with differentiation degree of oste osarcomas,so was with the sex of the p atient.
