本文克隆了RIN4(RPM1-interacting protein 4)在胡杨中的同源基因PeRIN4,并在拟南芥中进行过表达,通过研究转基因株系的耐盐表型、质膜H^+-ATPsae活性及H^+、Na^+、K^+等的动态离子流,揭示了PeRIN4基因在植物响应和适应盐胁迫环境中的...本文克隆了RIN4(RPM1-interacting protein 4)在胡杨中的同源基因PeRIN4,并在拟南芥中进行过表达,通过研究转基因株系的耐盐表型、质膜H^+-ATPsae活性及H^+、Na^+、K^+等的动态离子流,揭示了PeRIN4基因在植物响应和适应盐胁迫环境中的作用。利用定位载体p Green0029-PeRIN4-GFP瞬时转化拟南芥叶肉细胞原生质体的方法,对胡杨PeRIN4蛋白进行亚细胞定位,发现该蛋白定位在细胞的胞质中。耐盐表型实验结果显示,在100 mmol/L NaCl处理下,拟南芥PeRIN4过表达株系(OE1和OE8)的生存率和根长均明显高于野生型(WT)和转空载体拟南芥(VC),说明PeRIN4基因能够提高拟南芥的耐盐性。与WT和VC相比,拟南芥PeRIN4过表达株系质膜H^+-ATPsae的活性较高。动态离子流数据显示,在盐胁迫下,PeRIN4过表达株系外排H^+和Na^+离子的能力强于野生型和转空载体拟南芥,然而K+的外流却弱于WT和VC。因此,PeRIN4蛋白具有调节质膜H^+-ATPsae活性的功能。拟南芥质膜H^+-ATPsae活性的提高主要有两方面的作用:一是可以增强H+泵的质子动力势,驱动Na^+/H^+逆向转运蛋白,提高Na^+外排的能力;二是抑制质膜的去极化,减少K+离子通过去极化激活的外向型K^+通道(DA-KORCs)和非选择性阳离子通道(DA-NSCCs)外流,维持了K^+/Na^+平衡,从而提高PeRIN4转基因拟南芥的耐盐性。展开更多
Scientific knowledge of lunar lithologies was first acquired in the 1960s-1970s.The space race between the United States(U.S.)and Soviet Union has promoted numerous manned and robotic lunar exploration missions.Utiliz...Scientific knowledge of lunar lithologies was first acquired in the 1960s-1970s.The space race between the United States(U.S.)and Soviet Union has promoted numerous manned and robotic lunar exploration missions.Utilizing datasets from these missions,the first series of lunar geologic maps was prepared and published by the U.S.Geological Survey(USGS)The definition of lunar geological features in these maps was mostly based on morphological characteristics but lacked lithological constraints owing to the incompleteness of the compositional datasets avail-able.After two decades of silence,a new era of lunar exploration began in the 1990s when the Galileo spacecraft flew by the Moon during its gravity-assisted maneuvers.The very successful orbital missions,the Clementine and Lunar Prospector(LP),provided the first global geochemical and mineralogical(multispectral,gamma ray,neutron,etc.)datasets of the lunar surface.展开更多
文摘骨骼肌功能障碍是慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)的重要组成部分,它包括呼吸肌以及外周非呼吸肌的功能下降,具体表现为:肌肉体积减小,运动强度下降,运动耐力下降。骨骼肌功能障碍严重影响到COPD患者的生活质量及疾病的预后。但是目前COPD的骨骼肌功能障碍发生的具体机制尚不十分明确。研究证实叉形头转录蛋白因子-O(fork head transcr iption factor protein O,FOXO)在维持骨骼肌的功能中具有重要作用。现就COPD患者的骨骼肌障碍与FOXO的关系进行综述。
基金supported by the National Science and Technology Infrastructure Work Projects(2015FY210500)Key Research Program of Frontier Sciences,Chinese Academy of Sciences(QYZDY-SSW-DQC028)+5 种基金Strategic Priority Program of Chinese Academy of Sciences(XDB41000000)the National Natural Science Foundation of China(42102280,41972322,and 11941001)the Natural Science Foundation of Shandong Province(ZR2021QD016)the China Postdoctoral Science Foundation(2020M682164)the State Scholarship Fund(201706220310)。
文摘Scientific knowledge of lunar lithologies was first acquired in the 1960s-1970s.The space race between the United States(U.S.)and Soviet Union has promoted numerous manned and robotic lunar exploration missions.Utilizing datasets from these missions,the first series of lunar geologic maps was prepared and published by the U.S.Geological Survey(USGS)The definition of lunar geological features in these maps was mostly based on morphological characteristics but lacked lithological constraints owing to the incompleteness of the compositional datasets avail-able.After two decades of silence,a new era of lunar exploration began in the 1990s when the Galileo spacecraft flew by the Moon during its gravity-assisted maneuvers.The very successful orbital missions,the Clementine and Lunar Prospector(LP),provided the first global geochemical and mineralogical(multispectral,gamma ray,neutron,etc.)datasets of the lunar surface.
