消炎痛抑制hCG诱发家兔排卵的研究Ⅱ

采用扫描电镜观察,比较诱发家兔排卵过程中对照组与消炎痛组卵泡顶部上皮细胞的改变。看到对照组上皮细胞随着卵泡的发育而逐渐扁平化,微绒毛消失,细胞脱落或溶解,暴露出白膜的胶原纤维,最后发生排卵。而消炎痛组上皮细胞改变不明显。提示消炎痛阻制卵泡顶部上皮细胞内酶的释放可能是抗排卵的原因之一。

消炎痛抑制hCG诱发家兔排卵的研究Ⅲ

利用冷冻割断技术,使用扫描电镜观察家兔排卵过程的卵泡壁改变。发现随着排卵的临近,卵泡的白膜和外膜成纤维细胞及胶原纤维板出现溶解、水肿,卵泡顶部内膜血管扩张充血和有凝血的发生。而注射消炎痛的家兔卵泡壁无明显水肿和溶解,内膜血管也无凝血。作者认为卵泡顶部内膜血管在排卵前的凝血是导致卵泡壁溶解而发生排卵的主要原因之一。

消炎痛抑制hCG诱发家兔排卵的研究Ⅰ

消炎痛能有效地抑制CG诱发家兔排卵。采用放免分析动态测定循环血中的孕酮和睾酮,证实消炎痛虽然阻抑了排卵,但不影响甾体性激素的代谢。提示非甾体抗炎药可能是有开发价值的避孕药。

The Observation of Blood-Uterus,-Oviduct and-Fimbriae Lumen Barriers Under Transmission Electron Microscope

The author has observed the epithelium and basement membrane of uterus, oviduct and fimbriae in mice under the TEM by oral administration of AgNo3 tracer. The results showed that there was obvious deposit of argentum granules outside the basement membrane of mucosal epithelium of the uterus, oviduct and fimbriae as well as outside the basement membrane of uterine glands. And there was dilatation fully filled with high electron-dense argentum granules at the intercellular space just beneath the tight junction between the mucosal epithelial cells of uterus, oviduet, fimbriae. All these finding suggest that the uterus, oviduct and fimbriae serve as barriers to the heavy metal argentum ions.

损伤脑垂体对小鼠子宫内膜T淋巴细胞亚群影响的实验研究

采用免疫组化荧光单克隆抗体方法 ,酶组化及透射电镜等方法对损伤脑垂体 (P组 )、腹腔注射HCG组 (H组 )、正常对照组 (N组 )小鼠子宫内膜 T淋巴细胞亚群的分布及变化进行研究。结果显示 :在小鼠正常动情周期内 ,子宫内膜 CD4/CD8比值动情期为 0 .35± 0 .15 ,动情前期 0 .5 4± 0 .13,间期则几乎见不到阳性细胞 ;P组小鼠子宫内膜 CD4/CD8比值为 1.0 7± 0 .15 ;而 H组小鼠的比值则明显下降 ,并且 ,P组小鼠子宫内膜出现明显的结构及酶活性的变化。综上结果提示 :小鼠正常动情周期内子宫内膜 T淋巴细胞亚群的分布有变化 ,其变化与激素水平相关 ;损伤脑垂体可致子宫内膜 CD4/CD8比值的明显升高 ,且对结构及功能有显著影响 ,以致子宫内膜局部正常免疫机制的改变。