乙型肝炎肝损害主要由细胞免疫反应所致，效应细胞主要是特异性细胞毒性 T 淋巴细胞（CTL）［1］。可溶性白细胞介素-2受体（sIL-2R）因其与免疫调节关系密切，加之取材方便，易于检测，现已作为临床衡量体内免疫功能的一种有用的新方法...乙型肝炎肝损害主要由细胞免疫反应所致，效应细胞主要是特异性细胞毒性 T 淋巴细胞（CTL）［1］。可溶性白细胞介素-2受体（sIL-2R）因其与免疫调节关系密切，加之取材方便，易于检测，现已作为临床衡量体内免疫功能的一种有用的新方法［2］。甘草酸制剂是目前临床上应用比较广泛的一类降酶护肝药物，异甘草酸镁是甘草酸单一立体异构体镁盐，具有较强的抗炎、保护肝细胞膜、解毒、抗生物氧化及改善肝功能作用［3，4］，MgIG 是第4代甘草酸制剂，对多种肝炎都具有良好效果，临床上广泛使用。本文通过检测慢性乙型肝炎患者血清slL-2R 和球蛋白等血清学指标水平，以及治疗过程中这些指标的动态变化，探讨异甘草酸镁在慢性乙型肝炎中的免疫调节作用。展开更多
HBV-associated acute-on-chronic liver failure is prevalent in China's Mainland. The prognosis of HBV-ACLF is poor. The mortality of HBV-ACLF is approximately 80%. Therefore, a prognostic indicator was needed in or...HBV-associated acute-on-chronic liver failure is prevalent in China's Mainland. The prognosis of HBV-ACLF is poor. The mortality of HBV-ACLF is approximately 80%. Therefore, a prognostic indicator was needed in order to allow us to intervene as soon as possible. The model for end-stage liver disease（MELD） scoring system is widely used to predict the prognosis of liver failure. However, the assessment is too complex to restrict its application. This study aimed to investigate the expression of IP-10 in peripheral blood mononuclear cells（PBMC）, in order to explore the relationship between the expression and prognosis of patients with HBV-ACLF. The mRNA level of IP-10 in PBMCs were analyzed in 80 patients with HBV-ACLF, 40 patients with chronic hepatitis B（CHB） and 40 healthy people by fluorescent quantitative PCR. IP-10 mRNA level was significantly higher in the HBV-ACLF group than in the other two groups（P〈0.01）. Group with MELD score below 30 had lower IP-10 mRNA level than group with MELD score over 30（P〈0.05）. The IP-10 mR NA level in PBMCs in positive group was higher than that in negative group（P〈0.01）. With a threshold of 0.925, the area under the receiver operating characteristic（ROC） curves was 0.815. These findings suggest that assessment of IP-10 mRNA level in the PBMCs would be helpful for evaluating the disease severity and prognosis in patients with HBV-ACLF.展开更多
文摘乙型肝炎肝损害主要由细胞免疫反应所致，效应细胞主要是特异性细胞毒性 T 淋巴细胞（CTL）［1］。可溶性白细胞介素-2受体（sIL-2R）因其与免疫调节关系密切，加之取材方便，易于检测，现已作为临床衡量体内免疫功能的一种有用的新方法［2］。甘草酸制剂是目前临床上应用比较广泛的一类降酶护肝药物，异甘草酸镁是甘草酸单一立体异构体镁盐，具有较强的抗炎、保护肝细胞膜、解毒、抗生物氧化及改善肝功能作用［3，4］，MgIG 是第4代甘草酸制剂，对多种肝炎都具有良好效果，临床上广泛使用。本文通过检测慢性乙型肝炎患者血清slL-2R 和球蛋白等血清学指标水平，以及治疗过程中这些指标的动态变化，探讨异甘草酸镁在慢性乙型肝炎中的免疫调节作用。
基金supported by Natural Science Foundation of Hubei Province(No.2013CFB385)
文摘HBV-associated acute-on-chronic liver failure is prevalent in China's Mainland. The prognosis of HBV-ACLF is poor. The mortality of HBV-ACLF is approximately 80%. Therefore, a prognostic indicator was needed in order to allow us to intervene as soon as possible. The model for end-stage liver disease（MELD） scoring system is widely used to predict the prognosis of liver failure. However, the assessment is too complex to restrict its application. This study aimed to investigate the expression of IP-10 in peripheral blood mononuclear cells（PBMC）, in order to explore the relationship between the expression and prognosis of patients with HBV-ACLF. The mRNA level of IP-10 in PBMCs were analyzed in 80 patients with HBV-ACLF, 40 patients with chronic hepatitis B（CHB） and 40 healthy people by fluorescent quantitative PCR. IP-10 mRNA level was significantly higher in the HBV-ACLF group than in the other two groups（P〈0.01）. Group with MELD score below 30 had lower IP-10 mRNA level than group with MELD score over 30（P〈0.05）. The IP-10 mR NA level in PBMCs in positive group was higher than that in negative group（P〈0.01）. With a threshold of 0.925, the area under the receiver operating characteristic（ROC） curves was 0.815. These findings suggest that assessment of IP-10 mRNA level in the PBMCs would be helpful for evaluating the disease severity and prognosis in patients with HBV-ACLF.
