溶栓剂的发展及研究

目的：探讨用于治疗血栓的溶栓剂的研究及发展的方向。方法：根据文献资料简述了血栓病、血栓形成及溶栓机制，比较了各种溶栓剂的优缺点，报告了溶栓剂的现状及发展。结果：应用现代分子生物学技术研制的新型溶栓剂，在特异性、半衰期及纤溶效率方面有较大提高。结论：导向溶栓剂是溶栓剂的研究及发展的重要方向。

抗癌药物3——苯基——3——氮桥环三氧化锗基丙酸的合成及分析

从原料锗氯仿与肉桂酸开始合成了３ —苯基—３ —氯锗基丙酸、３ —苯基—３ —三甲氧基锗基丙酸及３ —苯基—３ —氮桥环三氧化锗基丙酸．用 Ｉ Ｒ，１ Ｈ Ｎ Ｍ Ｒ 及１３ Ｃ Ｎ Ｍ Ｒ

从猪胸腺提取胸腺素1的工艺

为了提高猪胸腺的利用价值,探索猪胸腺素活性物质的提取工艺。选取猪胸腺为原料,采用匀浆、凝胶过滤、离子交换层析和丙酮分级沉淀的方法纯化胸腺素α1,并用Bradford法检测蛋白含量,用SDS-PAGE电泳测定蛋白纯度。将猪胸腺组织匀浆、三次冻融和80℃热变性纯化后,上清采用Sephadex G-50柱填料凝胶过滤,然后进行阴离子交换树脂DEAE-C32离子交换层析和丙酮分级沉淀,通过真空冷冻干燥,最后得到胸腺素α1,纯度为95%以上,得率为3.84×10-5。最终得到提取胸腺素α1的有效工艺。

Extraction and DNA Digestion of 5'-Phosphodiesterase from Malt Root

This study investigated the extraction of 5＇-phosphodiesterase from malt root and the degradation of nucleic acids by this enzyme. The extraction used grade precipitation with ammonium sulfate and enzymatic hydrolysis. Samples were assayed using the modified Bradford method and high performance liquid chromatography. The results show that 5＇-phosphodiesterase is isolated by grade precipitation with 30% and 80% saturation of ammonium sulfate and can be utilized to degrade deoxyribonucleic acid. The hydrolysate has four kinds of deoxynucleotides： 5＇-dCMP, 5＇-dTMP, 5＇-dAMP, and 5＇-dGMP. The optimum reaction temperature is 70℃, and the optimum pH is 5.5-6.0 for the reaction. The percentage of deoxynucleotides indicated by the China Pharmacopoeia （2000 edition） in the product is over 70%. The extraction of 5＇-phosphodiesterase from malt root is shown to be possible and economical. Products from the enzYmatic hydrolysate of DNA meet the pharmacopoeia.