This study examined the effect of IKVAV peptide nanofiber on proliferation, adhesion and differentiation into neurocytes of bone marrow stromal cells （BMSCs）. IKVAV Peptide-amphiphile was synthesized and purified. T...This study examined the effect of IKVAV peptide nanofiber on proliferation, adhesion and differentiation into neurocytes of bone marrow stromal cells （BMSCs）. IKVAV Peptide-amphiphile was synthesized and purified. Then, hydrogen chloride was added to the diluted aqueous solutions of PA to induce spontaneous formation of nanofiber in vitro. The resultant samples was observed tmder transmission electron microscope. BMSCs were cultured with IKVAV peptide nanofiber. The effect of IKVAV nanofiber on the proliferation, adhesion and induction differentiation of BMSCs was observed by inverted microscopy, calcein-AM/PI staining, cell counting and immunofluorescence staining. The results demonstrated that IKVAV peptide-amphiphile could self-assemble to form nanofiber gel. BMSCs cultured in combination with IKVAV peptide nanofiber gel grew well and the percentage of live cells was over 90%. IKVAV peptide nanofiber gel exerted no influence on the proliferation of BMSCs and could promote the adhesion of BMSCs and raise the ra- tio of neurons when BMSCs were induced to differentiate into neurocytes. It is concluded that BMSCs could proliferate and adhere well and yield more neurons during when induced to differente into neurocytes on IKVAV peptide nanofiber gel.展开更多
The factors influencing the incidence of common complications（pneumothorax and pulmonary hemorrhage） of CT-guided percutaneous needle biopsy of lumps near pulmonary hilum were investigated. CT-guided percutaneous ne...The factors influencing the incidence of common complications（pneumothorax and pulmonary hemorrhage） of CT-guided percutaneous needle biopsy of lumps near pulmonary hilum were investigated. CT-guided percutaneous needle biopsy of lumps near pulmonary hilum was performed on 48 patients. The complications of pneumothorax and pneumorrhagia as well as the contributing factors were analyzed statistically. The major complications associated with CT-guided needle biopsy included pneumothorax（13 cases, 27.1%） and pulmonary hemorrhage（14 cases, 20.24%）. ？2 test revealed that pneumothorax was associated with the lesion size and depth of needle penetration, and pulmonary hemorrhage with the depth of needle penetration and needle retention time with a significant P value. Pneumothorax was observed in 7 cases（17.5%） out of 40 cases with diameter of mass greater than 3 cm, and in 6 cases（60%） out of 10 cases with depth of needle penetration greater than 4 cm. Additionally, pulmonary hemorrhage was identified in 12 cases（41.4%） out of 29 cases with needle retention time longer than 15 min, and pulmonary hemorrhage in 7 cases（70%） out of 10 cases with depth of needle penetration greater than 4 cm. CT-guided percutaneous needle biopsy of lumps near pulmonary hilum is safe and effective. The key factors to prevent the complications include correct evaluation of lesion size, depth of needle penetration and the needle retention time before the operation. Key words： biopsy, CT-guided; hilum; pneumothorax; pulmonary hemorrhage展开更多
Summary： The purpose of the present study was to observe the structure and functional change of the bone-coating-prosthesis interface in vivo and to evaluate the histocompatibility of self-made prosthetic femoral com...Summary： The purpose of the present study was to observe the structure and functional change of the bone-coating-prosthesis interface in vivo and to evaluate the histocompatibility of self-made prosthetic femoral components in the body and the degree of their bonding with the surrounding bone tissues as well as their stability. Six mature beagle dogs underwent bilateral hip replacement with prosthetic femur components. Three groups were established in terms of different coating of prothesis （four joints in each group）： atmosphere （A） plasma-sprayed pure titanium （Ti） prosthetic joint with hydroxyapatite （HA） coating （HA＋Ti＋A group）; vacuum （V） plasma-sprayed pure Ti prosthetic joint with HA coating （HA＋Ti＋V group）; vacuum plasma-sprayed pure Ti prosthetic joint with Ti-HA stepped coating （Ti＋HAG＋Ti＋V group）. The hip joints were functionally evaluated, and subjected to X-ray examination, biomechanics inspection, and histological examination. As a result, X-ray imaging revealed all prosthetic joints were in a good location and no dislocation of joint was found. Shear strength of interface was significantly higher in Ti＋HAG＋Ti＋V group than in HA＋Ti＋V group （P〈0.05） and HA＋Ti＋A group （P〈0.05） at 28th week. Histological examination showed the amount of newborn bone in Ti＋HAG＋Ti＋V group was more than in HA＋Ti＋V group and HA＋Ti＋A group after 28 weeks. It was suggested that vacuum plasma-sprayed pure Ti prosthetic joint with TI-HA stepped coating could improve the bonding capacity of bone-prosthesis, enhance the stability of prosthesis, and increase the fixion of prosthetic femoral components because of better bone growth. This new type of biological material in prosthetic femoral components holds promises for application in clinical practice.展开更多
目的观察自组装含IKVAV多肽凝胶材料与神经组织的生物相容性。方法肽溶于NaOH溶液中，调整pH至8．5，浓度为0．01g／ml，加DMEM／F12在盖玻片上触发多肽自组装为凝胶，透射电镜检测。取新生SD大鼠背根神经节及其游离细胞分为实验组与对...目的观察自组装含IKVAV多肽凝胶材料与神经组织的生物相容性。方法肽溶于NaOH溶液中，调整pH至8．5，浓度为0．01g／ml，加DMEM／F12在盖玻片上触发多肽自组装为凝胶，透射电镜检测。取新生SD大鼠背根神经节及其游离细胞分为实验组与对照组，实验组中DRG及其游离细胞与凝胶材料联合培养。倒置相差显微镜观察DRG生长情况，免疫荧光染色结合镜下细胞计数检测凝胶材料的细胞毒性和黏附性。结果透射电镜显示：自组装凝胶为编织状纳米纤维。实验组中DRG轴突生长情况优于对照组。培养1、3d后，两组中活细胞比例差异元统计学意义。培养3、12 h 后，实验组中神经细胞黏附数显著高于对照组。结论含IKVAV多肽凝胶材料能促进DRG生长和神经细胞黏附，对细胞毒副作用小，可作为优良的神经组织工程支架材料。展开更多
基金supported by grants from the National Natural Sciences Foundation of China (No. 30500511)the National High-tech Research Program (No. 2006AA320 605)
文摘This study examined the effect of IKVAV peptide nanofiber on proliferation, adhesion and differentiation into neurocytes of bone marrow stromal cells （BMSCs）. IKVAV Peptide-amphiphile was synthesized and purified. Then, hydrogen chloride was added to the diluted aqueous solutions of PA to induce spontaneous formation of nanofiber in vitro. The resultant samples was observed tmder transmission electron microscope. BMSCs were cultured with IKVAV peptide nanofiber. The effect of IKVAV nanofiber on the proliferation, adhesion and induction differentiation of BMSCs was observed by inverted microscopy, calcein-AM/PI staining, cell counting and immunofluorescence staining. The results demonstrated that IKVAV peptide-amphiphile could self-assemble to form nanofiber gel. BMSCs cultured in combination with IKVAV peptide nanofiber gel grew well and the percentage of live cells was over 90%. IKVAV peptide nanofiber gel exerted no influence on the proliferation of BMSCs and could promote the adhesion of BMSCs and raise the ra- tio of neurons when BMSCs were induced to differentiate into neurocytes. It is concluded that BMSCs could proliferate and adhere well and yield more neurons during when induced to differente into neurocytes on IKVAV peptide nanofiber gel.
基金supported by the National Natural Science Foundation of China(No.81102074)
文摘The factors influencing the incidence of common complications（pneumothorax and pulmonary hemorrhage） of CT-guided percutaneous needle biopsy of lumps near pulmonary hilum were investigated. CT-guided percutaneous needle biopsy of lumps near pulmonary hilum was performed on 48 patients. The complications of pneumothorax and pneumorrhagia as well as the contributing factors were analyzed statistically. The major complications associated with CT-guided needle biopsy included pneumothorax（13 cases, 27.1%） and pulmonary hemorrhage（14 cases, 20.24%）. ？2 test revealed that pneumothorax was associated with the lesion size and depth of needle penetration, and pulmonary hemorrhage with the depth of needle penetration and needle retention time with a significant P value. Pneumothorax was observed in 7 cases（17.5%） out of 40 cases with diameter of mass greater than 3 cm, and in 6 cases（60%） out of 10 cases with depth of needle penetration greater than 4 cm. Additionally, pulmonary hemorrhage was identified in 12 cases（41.4%） out of 29 cases with needle retention time longer than 15 min, and pulmonary hemorrhage in 7 cases（70%） out of 10 cases with depth of needle penetration greater than 4 cm. CT-guided percutaneous needle biopsy of lumps near pulmonary hilum is safe and effective. The key factors to prevent the complications include correct evaluation of lesion size, depth of needle penetration and the needle retention time before the operation. Key words： biopsy, CT-guided; hilum; pneumothorax; pulmonary hemorrhage
基金supported by the Natural Science Foundation of Hubei Province of China(No.2012FFB02431)
文摘Summary： The purpose of the present study was to observe the structure and functional change of the bone-coating-prosthesis interface in vivo and to evaluate the histocompatibility of self-made prosthetic femoral components in the body and the degree of their bonding with the surrounding bone tissues as well as their stability. Six mature beagle dogs underwent bilateral hip replacement with prosthetic femur components. Three groups were established in terms of different coating of prothesis （four joints in each group）： atmosphere （A） plasma-sprayed pure titanium （Ti） prosthetic joint with hydroxyapatite （HA） coating （HA＋Ti＋A group）; vacuum （V） plasma-sprayed pure Ti prosthetic joint with HA coating （HA＋Ti＋V group）; vacuum plasma-sprayed pure Ti prosthetic joint with Ti-HA stepped coating （Ti＋HAG＋Ti＋V group）. The hip joints were functionally evaluated, and subjected to X-ray examination, biomechanics inspection, and histological examination. As a result, X-ray imaging revealed all prosthetic joints were in a good location and no dislocation of joint was found. Shear strength of interface was significantly higher in Ti＋HAG＋Ti＋V group than in HA＋Ti＋V group （P〈0.05） and HA＋Ti＋A group （P〈0.05） at 28th week. Histological examination showed the amount of newborn bone in Ti＋HAG＋Ti＋V group was more than in HA＋Ti＋V group and HA＋Ti＋A group after 28 weeks. It was suggested that vacuum plasma-sprayed pure Ti prosthetic joint with TI-HA stepped coating could improve the bonding capacity of bone-prosthesis, enhance the stability of prosthesis, and increase the fixion of prosthetic femoral components because of better bone growth. This new type of biological material in prosthetic femoral components holds promises for application in clinical practice.
文摘目的观察自组装含IKVAV多肽凝胶材料与神经组织的生物相容性。方法肽溶于NaOH溶液中，调整pH至8．5，浓度为0．01g／ml，加DMEM／F12在盖玻片上触发多肽自组装为凝胶，透射电镜检测。取新生SD大鼠背根神经节及其游离细胞分为实验组与对照组，实验组中DRG及其游离细胞与凝胶材料联合培养。倒置相差显微镜观察DRG生长情况，免疫荧光染色结合镜下细胞计数检测凝胶材料的细胞毒性和黏附性。结果透射电镜显示：自组装凝胶为编织状纳米纤维。实验组中DRG轴突生长情况优于对照组。培养1、3d后，两组中活细胞比例差异元统计学意义。培养3、12 h 后，实验组中神经细胞黏附数显著高于对照组。结论含IKVAV多肽凝胶材料能促进DRG生长和神经细胞黏附，对细胞毒副作用小，可作为优良的神经组织工程支架材料。
