目的全面收集国内外立体定向放射治疗(SBRT)和射频消融(RFA)治疗肝细胞癌(HCC)相关研究文献,通过Meta分析系统比较这两种治疗方法的临床效果及相关并发症。方法计算机检索PubMed、Embase、Cochrane Library、Scopus、Web of Science以...目的全面收集国内外立体定向放射治疗(SBRT)和射频消融(RFA)治疗肝细胞癌(HCC)相关研究文献,通过Meta分析系统比较这两种治疗方法的临床效果及相关并发症。方法计算机检索PubMed、Embase、Cochrane Library、Scopus、Web of Science以及中国生物医学文献(CBM)、知网、万方、维普等中英文数据库自建库至2022年6月发表的SBRT和RFA临床治疗HCC文献。采用Stata 14.0软件进行Meta分析。结果共纳入14项回顾性研究6806例患者。基于总生存期(OS)的综合风险比(HR)结果显示SBRT术后OS低于RFA术后(HR=1.25,95%CI=1.10~1.43,I^(2)=0%,P=0.0009),而局部控制(LC)比率的综合HR显示SBRT有更好的疗效(HR=0.61,95%CI=0.47~0.78,I^(2)=0%,P=0.0001)。亚组分析显示,LC比率的综合HR更支持对肿瘤直径>2 cm患者行SBRT(HR=2.64,95%CI=1.56~4.48,I^(2)=0%,P=0.0003)。SBRT组与RFA组间晚期严重不良反应发生率差异无统计学意义(OR=1.01,95%CI=0.59~1.73,I^(2)=30%,P=0.97)。结论SBRT与RFA相比虽未表现出生存获益上的优势,但在HCC局部控制方面表现出较优的效果,尤其是对肿瘤直径>2 cm的患者。展开更多
Various kinds of schiff base metal complexes have been proven to induce apoptosis of tumor cells. However,it remains largely unknown whether schiff base zinc complexes induce apoptosis in human cancer cells. Here,we s...Various kinds of schiff base metal complexes have been proven to induce apoptosis of tumor cells. However,it remains largely unknown whether schiff base zinc complexes induce apoptosis in human cancer cells. Here,we synthesized a novel schiff base zinc coordination compound（SBZCC） and investigated its effects on the growth,proliferation and apoptosis of human osteosarcoma MG-63 cells. A novel SBZCC was synthesized by chemical processes and used to treat MG-63 cells. The cell viability was determined by CCK-8 assay. The cell cycle progression,mitochondrial membrane potential and apoptotic cells were analyzed by flow cytometry. The apoptosis-related proteins levels were determined by immunoblotting. Treatment of MG-63 cells with SBZCC resulted in inhibition of cell proliferation and cell cycle arrest at G1 phase. Moreover,SBZCC significantly reduced the mitochondrial membrane potential and induced apoptosis,accompanied with increased Bax/Bcl-2 and Flas L/Fas expression as well as caspase-3/8/9 cleavage. Our results demonstrated that the synthesized novel SBZCC could inhibit the proliferation and induce apoptosis of MG-63 cells via activating both the mitochondrial and cell death receptor apoptosis pathways,suggesting that SBZCC is a promising agent for the development as anticancer drugs.展开更多
基金supported by grants from the Scientific Research Foundation of Hebei Provincial Health Department,China(No.ZL20140116)the Clinical Research Special Fund of Wu Jieping Medical Foundation(No.320.6750.14119)
文摘Various kinds of schiff base metal complexes have been proven to induce apoptosis of tumor cells. However,it remains largely unknown whether schiff base zinc complexes induce apoptosis in human cancer cells. Here,we synthesized a novel schiff base zinc coordination compound（SBZCC） and investigated its effects on the growth,proliferation and apoptosis of human osteosarcoma MG-63 cells. A novel SBZCC was synthesized by chemical processes and used to treat MG-63 cells. The cell viability was determined by CCK-8 assay. The cell cycle progression,mitochondrial membrane potential and apoptotic cells were analyzed by flow cytometry. The apoptosis-related proteins levels were determined by immunoblotting. Treatment of MG-63 cells with SBZCC resulted in inhibition of cell proliferation and cell cycle arrest at G1 phase. Moreover,SBZCC significantly reduced the mitochondrial membrane potential and induced apoptosis,accompanied with increased Bax/Bcl-2 and Flas L/Fas expression as well as caspase-3/8/9 cleavage. Our results demonstrated that the synthesized novel SBZCC could inhibit the proliferation and induce apoptosis of MG-63 cells via activating both the mitochondrial and cell death receptor apoptosis pathways,suggesting that SBZCC is a promising agent for the development as anticancer drugs.
