遗传多样性的精准鉴定是资源可持续利用的基础。我们利用松属的3个种的叶绿体基因组序列中的物种特有的535个单核苷酸多态位点作为分子性状首次编制分子鉴定检索表,供试样品得到成功鉴定。物种特有的单核苷酸多态位点的数量和核苷酸构...遗传多样性的精准鉴定是资源可持续利用的基础。我们利用松属的3个种的叶绿体基因组序列中的物种特有的535个单核苷酸多态位点作为分子性状首次编制分子鉴定检索表,供试样品得到成功鉴定。物种特有的单核苷酸多态位点的数量和核苷酸构成存在种间差异。马尾松Pinus massoniana Lamb. (252)的特有单核苷酸多态位点的数量最多,随后依次是赤松P. densiflora Siebold & Zucc. (175)和黑松P. thunbergii Parl. (108)。马尾松的特有单核苷酸多态位点中,T的比例(29.37%)最高,随后依次是C (25.79%)、A (22.62%)和G (22.22%),G的比例最低,总体上差异较小。黑松的特有单核苷酸多态位点中,T (36.11%)的比例较C (12.96%)的比例高2.7倍,较G (20.37%)的比例高1.7倍;A (30.56%)的比例略低于T的比例,是G的1.5倍,是C的2.3倍。赤松的特有单核苷酸多态位点中,T (31.43%)和A (28.57%)的比例明显高于C (18.86%)或G (21.14%)的比例。结果显示,叶绿体基因组的单核苷酸多态位点信息,可用于松属植物资源遗传多样性的分子鉴定。调查了中国过去120多年来松属植物标本的采集和馆藏现状,讨论了存在的问题和对策。本研究对于松属植物的分类修订、种质资源的保护与利用具有重要价值。Accurate identification of genetic diversity is essential for sustainable utilization of plant resources. In this paper, 535 single nucleotide polymorphism (SNP) sites in the chloroplast genomes of 3 species from the genus Pinus L. were used as molecular traits to identify the plant genetic resource diversity of this plant genus and to compile a molecular classification key for the first time. There are differences in aspects of amount and base composition of SNPs among the species. The amount of SNPs in Pinus massoniana Lamb. (252) is the highest, being higher than that in P. densiflora Siebold & Zucc. (175) or P. thunbergii Parl. (108). In P. massoniana, the proportion of T (29.37%) is the highest, larger than that of C (25.79), and that of A (22.62%) or G (22.62%) is the lowest. In P. thunbergii, the proportion of T (36.11%) is 2.7 times that of C (12.96%), and 1.7 times that of G (20.37%). A (30.56%) is a little bit smaller than that of T, being 1.5 times that of G, and 2.3 times that of C. In P. densiflora, the proportion of T (31.43%) or A (28.57%) is significantly higher than that of C (18.86%) or G (21.14%). Our results indicated that single nucleotide polymorphism sites from the chloroplast genomes could be used for distinguishing different species successfully in the genus Pinus. The status of Pinus plant specimens collected in the past over 120 years in China is investigated, and problems and possible strategies are discussed. This study is valuable for taxonomic revision, conservation and utilization of Pinus plant germplasm resources.展开更多
The techniques of cutting propagation of Potentilla fruticosa was studied.The tender stem cuttings and the woody stem cuttings were treated by different concentrations of BBA(0 mg·kg-1,10 mg·kg-1,50 mg·...The techniques of cutting propagation of Potentilla fruticosa was studied.The tender stem cuttings and the woody stem cuttings were treated by different concentrations of BBA(0 mg·kg-1,10 mg·kg-1,50 mg·kg-1,100 mg·kg-1,200 mg·kg-1,500 mg·kg-1).The results indicated that the rooting percentage of tender stem cuttings at the concentration of 10 mg ·kg-1BBA was the highest(88.3%),and that of woody stem cuttings treated by the concentration of 50 mg·kg-1BBA was the highest(66.67%).It also indicated that the rooting percentages of the tender stem cuttings were better than those of the woody stem cuttings with same treatment.展开更多
文摘遗传多样性的精准鉴定是资源可持续利用的基础。我们利用松属的3个种的叶绿体基因组序列中的物种特有的535个单核苷酸多态位点作为分子性状首次编制分子鉴定检索表,供试样品得到成功鉴定。物种特有的单核苷酸多态位点的数量和核苷酸构成存在种间差异。马尾松Pinus massoniana Lamb. (252)的特有单核苷酸多态位点的数量最多,随后依次是赤松P. densiflora Siebold & Zucc. (175)和黑松P. thunbergii Parl. (108)。马尾松的特有单核苷酸多态位点中,T的比例(29.37%)最高,随后依次是C (25.79%)、A (22.62%)和G (22.22%),G的比例最低,总体上差异较小。黑松的特有单核苷酸多态位点中,T (36.11%)的比例较C (12.96%)的比例高2.7倍,较G (20.37%)的比例高1.7倍;A (30.56%)的比例略低于T的比例,是G的1.5倍,是C的2.3倍。赤松的特有单核苷酸多态位点中,T (31.43%)和A (28.57%)的比例明显高于C (18.86%)或G (21.14%)的比例。结果显示,叶绿体基因组的单核苷酸多态位点信息,可用于松属植物资源遗传多样性的分子鉴定。调查了中国过去120多年来松属植物标本的采集和馆藏现状,讨论了存在的问题和对策。本研究对于松属植物的分类修订、种质资源的保护与利用具有重要价值。Accurate identification of genetic diversity is essential for sustainable utilization of plant resources. In this paper, 535 single nucleotide polymorphism (SNP) sites in the chloroplast genomes of 3 species from the genus Pinus L. were used as molecular traits to identify the plant genetic resource diversity of this plant genus and to compile a molecular classification key for the first time. There are differences in aspects of amount and base composition of SNPs among the species. The amount of SNPs in Pinus massoniana Lamb. (252) is the highest, being higher than that in P. densiflora Siebold & Zucc. (175) or P. thunbergii Parl. (108). In P. massoniana, the proportion of T (29.37%) is the highest, larger than that of C (25.79), and that of A (22.62%) or G (22.62%) is the lowest. In P. thunbergii, the proportion of T (36.11%) is 2.7 times that of C (12.96%), and 1.7 times that of G (20.37%). A (30.56%) is a little bit smaller than that of T, being 1.5 times that of G, and 2.3 times that of C. In P. densiflora, the proportion of T (31.43%) or A (28.57%) is significantly higher than that of C (18.86%) or G (21.14%). Our results indicated that single nucleotide polymorphism sites from the chloroplast genomes could be used for distinguishing different species successfully in the genus Pinus. The status of Pinus plant specimens collected in the past over 120 years in China is investigated, and problems and possible strategies are discussed. This study is valuable for taxonomic revision, conservation and utilization of Pinus plant germplasm resources.
文摘The techniques of cutting propagation of Potentilla fruticosa was studied.The tender stem cuttings and the woody stem cuttings were treated by different concentrations of BBA(0 mg·kg-1,10 mg·kg-1,50 mg·kg-1,100 mg·kg-1,200 mg·kg-1,500 mg·kg-1).The results indicated that the rooting percentage of tender stem cuttings at the concentration of 10 mg ·kg-1BBA was the highest(88.3%),and that of woody stem cuttings treated by the concentration of 50 mg·kg-1BBA was the highest(66.67%).It also indicated that the rooting percentages of the tender stem cuttings were better than those of the woody stem cuttings with same treatment.
