A method for rapid quantitative determination of sucrose in isatis-root granules by Fourier Transform Infrared Spectrophotometry(FTIR) was developed.Potassium ferricyanide was chosen as an inside mark material because...A method for rapid quantitative determination of sucrose in isatis-root granules by Fourier Transform Infrared Spectrophotometry(FTIR) was developed.Potassium ferricyanide was chosen as an inside mark material because it has only one strong absorption peak at 2117cm-1 and the peak was chosen as its quantitative peak.The peak at 1283cm-1 is the characteristic absorption of sucrose and was chosen as the quantitative peak of sucrose.The ratio of the two absorbances is I=Ai/As.K3[Fe(CN)6] and sucrose were mixed according to a certain mass ratio m=mi/ms to prepare standard samples.The curve of m versus I is the quantitative working curve.The linear range for sucrose is 5.0～23.0mg/g,the recovery is in the range of 91%～103%,the relative standard deviation is less than 5%.展开更多
文摘A method for rapid quantitative determination of sucrose in isatis-root granules by Fourier Transform Infrared Spectrophotometry(FTIR) was developed.Potassium ferricyanide was chosen as an inside mark material because it has only one strong absorption peak at 2117cm-1 and the peak was chosen as its quantitative peak.The peak at 1283cm-1 is the characteristic absorption of sucrose and was chosen as the quantitative peak of sucrose.The ratio of the two absorbances is I=Ai/As.K3[Fe(CN)6] and sucrose were mixed according to a certain mass ratio m=mi/ms to prepare standard samples.The curve of m versus I is the quantitative working curve.The linear range for sucrose is 5.0～23.0mg/g,the recovery is in the range of 91%～103%,the relative standard deviation is less than 5%.
