Transposon Tn 233(CH) encoding resistance to streptomycin, sulfonamide and mercuric salt was originally resident on Shigella flexneri plasmid DR233. In order to study its molecular characters, Tn233(CH) was transposed...Transposon Tn 233(CH) encoding resistance to streptomycin, sulfonamide and mercuric salt was originally resident on Shigella flexneri plasmid DR233. In order to study its molecular characters, Tn233(CH) was transposed to plasmid pBR322, and展开更多
The complete nucleotide (nt) sequence of the rice waxy(Wx) gene, which is responsible for the synthesis of amylose in endosperm and pollen, has been determined by a combination of restriction mapping and nt sequence a...The complete nucleotide (nt) sequence of the rice waxy(Wx) gene, which is responsible for the synthesis of amylose in endosperm and pollen, has been determined by a combination of restriction mapping and nt sequence analysis of two overlapping genomic DNA clones. The entire gene is about 5.5 kb in length. The alignment of the nt sequence of the Wx gene from rice with those of maize (Klsgen, R. B. et al.) and barley (Rohde, W. et al.) revealed the presence of thirteen introns and fourteen exons. The full-length of Wx protein in cluding transit peptide is 609 amino acid (aa) residues. The calculated molecular weight of rice Wx preprotein is about 72 kD. There is no significant difference between the similarity scores of the aa sequence deduced from the rice Wx gene compared with those of maize and barley. However, the nt sequences of the 5'-end upstream, 3'-end downstream and introns of the rice Wx gene, as well as the aa sequence of the transit peptide region of the Wx preprotein have low similarity scores in comparison with those of maize and barley.展开更多
文摘Transposon Tn 233(CH) encoding resistance to streptomycin, sulfonamide and mercuric salt was originally resident on Shigella flexneri plasmid DR233. In order to study its molecular characters, Tn233(CH) was transposed to plasmid pBR322, and
基金Project supported by the grants from the High Technology Development Programme of China and the Rockefeller Foundation.
文摘The complete nucleotide (nt) sequence of the rice waxy(Wx) gene, which is responsible for the synthesis of amylose in endosperm and pollen, has been determined by a combination of restriction mapping and nt sequence analysis of two overlapping genomic DNA clones. The entire gene is about 5.5 kb in length. The alignment of the nt sequence of the Wx gene from rice with those of maize (Klsgen, R. B. et al.) and barley (Rohde, W. et al.) revealed the presence of thirteen introns and fourteen exons. The full-length of Wx protein in cluding transit peptide is 609 amino acid (aa) residues. The calculated molecular weight of rice Wx preprotein is about 72 kD. There is no significant difference between the similarity scores of the aa sequence deduced from the rice Wx gene compared with those of maize and barley. However, the nt sequences of the 5'-end upstream, 3'-end downstream and introns of the rice Wx gene, as well as the aa sequence of the transit peptide region of the Wx preprotein have low similarity scores in comparison with those of maize and barley.
