A study was conducted to evaluate the cultivable filamentous fungal diversity in organic layers (L, F, and H layers) and A1 layer of two main forest types, Pinus massoniana and Liguidambar formasana mixed forest and Q...A study was conducted to evaluate the cultivable filamentous fungal diversity in organic layers (L, F, and H layers) and A1 layer of two main forest types, Pinus massoniana and Liguidambar formasana mixed forest and Quercus variabilis forest, in Zijin Mountain(325?N, 11848?E), Nanjing, China. A total of 67 taxa comprising 56 Deuteromycetes, 3 Zygomycetes, 5 Asco-mycetes and 3 unidentified fungi were recognized from samples from the forest floor of the two forest types. The most abundant group was Deuteromycetes. The dominant genera in both forests were Alternaria sp., Aspergillus sp., Cladosporium sp., Mucor sp., Penicillium sp., Rhizopus sp., Gliocladium sp. and Trichoderma spp. The fungal diversity was higher in the mixed forest than that in Q. variabilis forest. For both forest types, the maximum fungal diversity was found in layer F and there existed significantly different in fungal diversity between layer F and layer L. In the mixed forest, richness of fungi isolated from needle litter (P. massoniana) was lower than that from leaf litter (L. formasana). The richness of fungi from needle litter increased with the in-crease of forest floor depth, but for leaf litter, the fungal diversity decreased with the depth of forest floor. The co-species of fungi from the two forest types, as well as from two kinds of litters in mixed forest, increased with the depth of the forest floor. The succession of fungi along with the process of decomposition was discussed here. The results also showed that litter quality was a critical factor affecting fungal diversity.展开更多
目的从何首乌Polygonum multiflorum中提取分离多糖,对其结构进行表征,并评价其免疫调节活性。方法采用水提、醇沉法从何首乌中提取粗多糖,经Q-Sepharose Fast Flow强阴离子交换色谱柱分离纯化得到何首乌多糖(PMT)。利用高效凝胶渗透色...目的从何首乌Polygonum multiflorum中提取分离多糖,对其结构进行表征,并评价其免疫调节活性。方法采用水提、醇沉法从何首乌中提取粗多糖,经Q-Sepharose Fast Flow强阴离子交换色谱柱分离纯化得到何首乌多糖(PMT)。利用高效凝胶渗透色谱-十八角度激光光散射联用法(HPGPC-MALLS)测定PMT的绝对分子质量,采用PMP柱前衍生-高效液相色谱法(HPLC)测定单糖组成。采用二维核磁共振波谱(2D-NMR)对PMT的结构进行表征。采用四甲偶氮唑盐(MTT)法、中性红比色法和Griess法分别检测PMT对小鼠单核巨噬细胞RAW264.7的生长、吞噬活性和释放一氧化氮(NO)能力的影响。结果 PMT是一种α-1,4-葡聚糖,其绝对分子质量为3.96×105。PMT可显著促进RAW264.7细胞的增殖,促进细胞的吞噬能力,增加NO的释放量。结论何首乌来源的α-1,4-葡聚糖具有显著的免疫调节活性,具有药物开发潜力。展开更多
基金This paper was supported by Chinese Program for High Technology Research and Development (2003AA209030) Scien-tific Research Foundation for doctoral supervising laboratory State Education Ministry (20030284044) and National Natural Sc
文摘A study was conducted to evaluate the cultivable filamentous fungal diversity in organic layers (L, F, and H layers) and A1 layer of two main forest types, Pinus massoniana and Liguidambar formasana mixed forest and Quercus variabilis forest, in Zijin Mountain(325?N, 11848?E), Nanjing, China. A total of 67 taxa comprising 56 Deuteromycetes, 3 Zygomycetes, 5 Asco-mycetes and 3 unidentified fungi were recognized from samples from the forest floor of the two forest types. The most abundant group was Deuteromycetes. The dominant genera in both forests were Alternaria sp., Aspergillus sp., Cladosporium sp., Mucor sp., Penicillium sp., Rhizopus sp., Gliocladium sp. and Trichoderma spp. The fungal diversity was higher in the mixed forest than that in Q. variabilis forest. For both forest types, the maximum fungal diversity was found in layer F and there existed significantly different in fungal diversity between layer F and layer L. In the mixed forest, richness of fungi isolated from needle litter (P. massoniana) was lower than that from leaf litter (L. formasana). The richness of fungi from needle litter increased with the in-crease of forest floor depth, but for leaf litter, the fungal diversity decreased with the depth of forest floor. The co-species of fungi from the two forest types, as well as from two kinds of litters in mixed forest, increased with the depth of the forest floor. The succession of fungi along with the process of decomposition was discussed here. The results also showed that litter quality was a critical factor affecting fungal diversity.
文摘目的从何首乌Polygonum multiflorum中提取分离多糖,对其结构进行表征,并评价其免疫调节活性。方法采用水提、醇沉法从何首乌中提取粗多糖,经Q-Sepharose Fast Flow强阴离子交换色谱柱分离纯化得到何首乌多糖(PMT)。利用高效凝胶渗透色谱-十八角度激光光散射联用法(HPGPC-MALLS)测定PMT的绝对分子质量,采用PMP柱前衍生-高效液相色谱法(HPLC)测定单糖组成。采用二维核磁共振波谱(2D-NMR)对PMT的结构进行表征。采用四甲偶氮唑盐(MTT)法、中性红比色法和Griess法分别检测PMT对小鼠单核巨噬细胞RAW264.7的生长、吞噬活性和释放一氧化氮(NO)能力的影响。结果 PMT是一种α-1,4-葡聚糖,其绝对分子质量为3.96×105。PMT可显著促进RAW264.7细胞的增殖,促进细胞的吞噬能力,增加NO的释放量。结论何首乌来源的α-1,4-葡聚糖具有显著的免疫调节活性,具有药物开发潜力。
