以α-和β-萘乙酸酯为底物,以固蓝 B 盐和 RR 盐为偶联剂,用聚丙烯酰胺垂直平板电泳技术研究了水稻酯酶同工酶的电泳显色,结果为①少数酶带只能用α-萘乙酸酯显示;绝大多数酶带可用α-或β-萘乙酸酯显示。②用α-和β-萘乙酸酯同时显色...以α-和β-萘乙酸酯为底物,以固蓝 B 盐和 RR 盐为偶联剂,用聚丙烯酰胺垂直平板电泳技术研究了水稻酯酶同工酶的电泳显色,结果为①少数酶带只能用α-萘乙酸酯显示;绝大多数酶带可用α-或β-萘乙酸酯显示。②用α-和β-萘乙酸酯同时显色时,一些单独用α-或β-萘乙酸酯能显示的酶带的相对染色强度或者降低(甚至酶带完全消失),或者增加。③用固蓝 B 盐作偶联剂与用固蓝 RR 盐相比,某些酶带的相对染色强度增加。因此,各电泳酶带染色强度的相对强弱并不一定反应各酶本身活性的相对高低。展开更多
Cytologic and embryologic studies on the reproductive pattern of male sterility line C1001 in rice (Oryza saliva L.) indicated that several types of apomixis were discovered in partial strains of male sterility line C...Cytologic and embryologic studies on the reproductive pattern of male sterility line C1001 in rice (Oryza saliva L.) indicated that several types of apomixis were discovered in partial strains of male sterility line C1001 in rice. Under a fully separate condition, two possible apomixis embryogenesis ways in the ovules were observed: One way was that the embryo was initiated from the somatic cells of the ovules, and then pushed against the embryo sac to develop into a mature embryo. The other way was that the embryo was from the unfertilized egg cell or one of synergids, and then developed into a mature embryo in the form of haploid parthenogenesis without forming endosperm. Besides, the antipodal cells migrated in the embryo sac, and their cytoplasm became thin and vacuolated while mitosis was taking place, giving rise to a multicellular structure or a group of large cells each containing several nuclei. This paper also discusses the significance and patterns of apomixis in rice C1001.展开更多
文摘以α-和β-萘乙酸酯为底物,以固蓝 B 盐和 RR 盐为偶联剂,用聚丙烯酰胺垂直平板电泳技术研究了水稻酯酶同工酶的电泳显色,结果为①少数酶带只能用α-萘乙酸酯显示;绝大多数酶带可用α-或β-萘乙酸酯显示。②用α-和β-萘乙酸酯同时显色时,一些单独用α-或β-萘乙酸酯能显示的酶带的相对染色强度或者降低(甚至酶带完全消失),或者增加。③用固蓝 B 盐作偶联剂与用固蓝 RR 盐相比,某些酶带的相对染色强度增加。因此,各电泳酶带染色强度的相对强弱并不一定反应各酶本身活性的相对高低。
文摘Cytologic and embryologic studies on the reproductive pattern of male sterility line C1001 in rice (Oryza saliva L.) indicated that several types of apomixis were discovered in partial strains of male sterility line C1001 in rice. Under a fully separate condition, two possible apomixis embryogenesis ways in the ovules were observed: One way was that the embryo was initiated from the somatic cells of the ovules, and then pushed against the embryo sac to develop into a mature embryo. The other way was that the embryo was from the unfertilized egg cell or one of synergids, and then developed into a mature embryo in the form of haploid parthenogenesis without forming endosperm. Besides, the antipodal cells migrated in the embryo sac, and their cytoplasm became thin and vacuolated while mitosis was taking place, giving rise to a multicellular structure or a group of large cells each containing several nuclei. This paper also discusses the significance and patterns of apomixis in rice C1001.
