The conformational changes,followed by fluorescence emission and exposure of buried SH groups,and the inactivation of lactate dehydrogenase M 4 in urea solutions have been studied. The results show that inactivation ...The conformational changes,followed by fluorescence emission and exposure of buried SH groups,and the inactivation of lactate dehydrogenase M 4 in urea solutions have been studied. The results show that inactivation occurs before noticeable conformational change can be detected.It can be suggested that the active sites of lactate dehydrogenase M 4 also display more conformational flexibility than the enzyme molecule as a whole.展开更多
文摘The conformational changes,followed by fluorescence emission and exposure of buried SH groups,and the inactivation of lactate dehydrogenase M 4 in urea solutions have been studied. The results show that inactivation occurs before noticeable conformational change can be detected.It can be suggested that the active sites of lactate dehydrogenase M 4 also display more conformational flexibility than the enzyme molecule as a whole.
