药物对豚鼠低氧灌注后肺动脉产生的氧自由基的清除效应

本实验采用电子自旋共振（ＥＳＲ）技术检测低氧灌注后的肺动脉氧自由基信号，同时观察超氧化物歧化酶（ＳＯＤ）和过氧化氢酶（Ｃａｔａｌａｓｅ），丹参对氧自由基信号的影响．豚鼠肺动脉，Ｋｒｅｂ＇ｓ营养液灌注，９５％Ｎ２＋５％ＣＯ２取代９５％Ｏ２＋５％ＣＯ２充气以产生低氧。灌注后的动脉经液氮冷冻，７７Ｋ，Ｘ波段，微波功率２０ｍＷ，１００Ｈｚ高频调制下作ＥＳＲ检测。测得低氧灌注的肺动脉有明显的氧自由基信号，ＳＯＤ，Ｃａｔａｌａｓｅ，丹参均能拮抗该信号．

芳酰基硒脲合成及其对O_2^-清除作用的EPR研究

芳酰基硒脲的合成试剂芳酰基异硒氰酸酯是极不稳定的化合物。通过硒氰化铅在非极性溶剂苯中同芳酰氯反应能简便、稳定和可靠地合成芳酰基异硒氰酸酯、从而再与芳胺反应能合成一系列芳酰基硒脲。经EPR研究其对超氧阴离子自由基O_2^-具有明显的清除作用。

β-酰胺基硒脲合成及其清除O_2^-作用EPR研究

硒从痕量元素到药物的研究重心的转移必将促使硒有机化合物药学即将诞生.硒代卡巴肼已被证实它具有抑制肿瘤生长和降低过氧化类酯的疗效、我们首次成功地合成了它的衍生物α-芳酰基β-酰胺基硒脲,经EPR研究证实它具有很强超氧阴离子自由基的捕获作用.

The Synthesis and EPR Study of Acylaminoselenoureasas O_2 Scavengel

With the center of research interest on selenium diverting from essential trace ele-ment to drug, birth of pharmacology of organo-selenium compounds will certainly beaccelerated. Selenosemi-carbazide has been demonstrated to have tumor-growthprohibiting and lipid-peroxidate lowering effects. We have for the first time success-fully synthesized its derivatives,α-aroyl β-acylamino selenoureas, and demonstrated byEPR study that they have very powerful O2 scavenger activity.