The effect of emulsification pretreatment on the acidic protease catalyzed hydrolysis of corn glu- ten reed(CGM)has been studied.Results show that under the hydrolysis conditions of acidic protease/sub- strate ratio o...The effect of emulsification pretreatment on the acidic protease catalyzed hydrolysis of corn glu- ten reed(CGM)has been studied.Results show that under the hydrolysis conditions of acidic protease/sub- strate ratio of 10%(g/g),CGM substrate concentration of 80 g/L and reaction temperature of 55℃,the di- rect hydrolysis of CGM by the acidic protease for 24 h gave a protein extraction rate of 62.79 % and a hydrol- ysate containing 45% oligo-peptides of molecular weight below 1000 u .By subjecting CGM under high- speed emulsification pretreatment at 7 000 r/min for 1 h,the hydrolysis of the resulted CGM fine particle un- der the same conditions gave a proptein extraction rate of 88.47% and a hydrolysate containing 78% oligo- peptides of molecular weight below 1000 u.The composition analysis of the hydrolysate on HPLC indicates that the emulsification pretreatment also increased the content of branch-chain amino acids in hydrolysate. That is to say,the emulsification pretreatment is useful for the preparation of high F ration oligo-peptides from CGM.展开更多
文摘The effect of emulsification pretreatment on the acidic protease catalyzed hydrolysis of corn glu- ten reed(CGM)has been studied.Results show that under the hydrolysis conditions of acidic protease/sub- strate ratio of 10%(g/g),CGM substrate concentration of 80 g/L and reaction temperature of 55℃,the di- rect hydrolysis of CGM by the acidic protease for 24 h gave a protein extraction rate of 62.79 % and a hydrol- ysate containing 45% oligo-peptides of molecular weight below 1000 u .By subjecting CGM under high- speed emulsification pretreatment at 7 000 r/min for 1 h,the hydrolysis of the resulted CGM fine particle un- der the same conditions gave a proptein extraction rate of 88.47% and a hydrolysate containing 78% oligo- peptides of molecular weight below 1000 u.The composition analysis of the hydrolysate on HPLC indicates that the emulsification pretreatment also increased the content of branch-chain amino acids in hydrolysate. That is to say,the emulsification pretreatment is useful for the preparation of high F ration oligo-peptides from CGM.
