Objective To investigate CD 80 expression on human tumor cell lines and establish stable CD 80 expression transfectants to illustrate CD 80 costimulation on the T cell proliferation and cytokine production. ...Objective To investigate CD 80 expression on human tumor cell lines and establish stable CD 80 expression transfectants to illustrate CD 80 costimulation on the T cell proliferation and cytokine production. Methods Raji, MDA 453, MCF 7, Hela, 3AO, MKN 45 and EBV transformed B cell were detected for CD 80 expression by RT PCR. CD 80 cDNA subcloned to retrovirus vector pLXSN, with them stable CD 80 transfectanants were established. With specific mAb BB1 and FACS assay, the expression of CD 80 were detected. Anti CD3 induced T lymphocyte proliferation and IL 2, IFN r production were performed to evaluate CD 80 costimulatory activity in the presence of calcium ionophore A23187 and phorbol ester PMA. Results CD 80 (B7 1) expression of MDA 453, MCF 7, Hela, 3AO, MKN 45 was negative, and that of Raji and EBV transformed B cell was positive by RT PCR and FACS methods. Cocultured with CD 80 transfected human breast carcinoma cell line MDA 453, anti CD3 induced T cells proliferation and cytokine production were significantly increased in the presence of A23187 and PMA, but not with parental MDA 453. Conclusions CD 80 expression is absent on most human tumor cell line except for Raji and EBV transformed B cell. Full activation of T cell needs CD 80 costimulation. Influence of CD 80 costimulation on cytokine production is mainly regulated via IL 2 and IFN r.展开更多
文摘Objective To investigate CD 80 expression on human tumor cell lines and establish stable CD 80 expression transfectants to illustrate CD 80 costimulation on the T cell proliferation and cytokine production. Methods Raji, MDA 453, MCF 7, Hela, 3AO, MKN 45 and EBV transformed B cell were detected for CD 80 expression by RT PCR. CD 80 cDNA subcloned to retrovirus vector pLXSN, with them stable CD 80 transfectanants were established. With specific mAb BB1 and FACS assay, the expression of CD 80 were detected. Anti CD3 induced T lymphocyte proliferation and IL 2, IFN r production were performed to evaluate CD 80 costimulatory activity in the presence of calcium ionophore A23187 and phorbol ester PMA. Results CD 80 (B7 1) expression of MDA 453, MCF 7, Hela, 3AO, MKN 45 was negative, and that of Raji and EBV transformed B cell was positive by RT PCR and FACS methods. Cocultured with CD 80 transfected human breast carcinoma cell line MDA 453, anti CD3 induced T cells proliferation and cytokine production were significantly increased in the presence of A23187 and PMA, but not with parental MDA 453. Conclusions CD 80 expression is absent on most human tumor cell line except for Raji and EBV transformed B cell. Full activation of T cell needs CD 80 costimulation. Influence of CD 80 costimulation on cytokine production is mainly regulated via IL 2 and IFN r.
