一种新的非酶法分离荠幼胚的方法

[目的]用非酶法手工分离荠的胚珠和幼胚等微小结构。[方法]应用胶块和解剖镜等普通工具,手工、非酶法分离并观察荠的花形态及荠胚珠和幼胚。[结果]荠花具有4片花萼、4片花瓣、四强雄蕊,短角果;荠的胚发育过程包括球形胚、心形胚和鱼雷形胚3个发育阶段。[结论]利用胶块的黏性,能使植物胚珠和胚等微小结构的手工分离操作变得比较简单,具有对幼胚损伤小、能保持原有形态等优点,可用于多领域的研究。该类工作在实验设施较差的实验室也能完成。

A New Non-enzymatic Method to Isolate Immature Embryos of Capsella bursa-pastoris (L.) Medic.

[Objective] The aim of this study was to manually isolate the microstructures such as ovule and embryo of Capsella bursa-pastoris (L.) Medic. by the non-enzymatic method. [Method] With the gum block and dissecting microscope, its floral characteristics, ovule and embryo were manually isolated and observed by the non-enzymatic method. [Result] C. bursa-pastoris had four calyces, four petals, tetradynamous stamen and silicle, while its embryogenesis contained globular embryo, heart-shaped embryo and torpedo-shaped embryo. [Conclusion] The microstructures such as ovule and embryo of plants become easier to be isolated manually with the viscosity of gum block, which has small damage on embryo or disadvantages of keeping original shapes, and also can used in multi fields studies. This test can also be accomplished in labs with poor experiment facilities.