Genomic DNA of Escherichia coli (belonging to Family Enterobacteriaccae, Genus Escherichia) strains K-12, B, C, 15 and derivatives of strain K-12 were used for comparison of their Sfi I restriction fragment patterns b...Genomic DNA of Escherichia coli (belonging to Family Enterobacteriaccae, Genus Escherichia) strains K-12, B, C, 15 and derivatives of strain K-12 were used for comparison of their Sfi I restriction fragment patterns by means of pulse field gel electrophoresis (PFGE). Results show that (i) Sfi I restriction fragment patterns of genomic DNA of strain K-12(wild type) and its derivatives(either lysogenic展开更多
A new type Ⅱ restriction endonuclease, BsrG I, has been isolated from Bacillusstearothermophilus GR75. BsrG I cleaves T7 DNA at 13 sites, Lambda DNA at
A new type Ⅱ restriction endonuclease, BsaO Ⅰ, has been isolated from the thermophilic bacillus Bacillus stearothermophilus O-122. This enzyme cleaves pBR322 DNA at 7 sites, pUC19 DNA at 5 sites and φ X 174 DNA at ...A new type Ⅱ restriction endonuclease, BsaO Ⅰ, has been isolated from the thermophilic bacillus Bacillus stearothermophilus O-122. This enzyme cleaves pBR322 DNA at 7 sites, pUC19 DNA at 5 sites and φ X 174 DNA at one site. The recognition sequence of BsaO Ⅰ was determined by mapping its cleavage sites on φ X 174 DNA to position 4600 and展开更多
When blood DNA was digested with Taq I and monitored for RFLP with a human cDNA probe from the 21-hydroxylase genes (pC21/3c)in our laboratory, besides two characteristic restriction fragments 3.2 kb and 3.7 kb, an ex...When blood DNA was digested with Taq I and monitored for RFLP with a human cDNA probe from the 21-hydroxylase genes (pC21/3c)in our laboratory, besides two characteristic restriction fragments 3.2 kb and 3.7 kb, an extra 5.6 kb band was revealed. This extra band appears both in normal Chinese individuals and in patients with congenital adrenal hyperplasia (CAH). The frequency is high (1/40 in phenotypically normal individuals and 1/18 in CAH patients). Evidently there is no selective pressure which is exerted展开更多
文摘Genomic DNA of Escherichia coli (belonging to Family Enterobacteriaccae, Genus Escherichia) strains K-12, B, C, 15 and derivatives of strain K-12 were used for comparison of their Sfi I restriction fragment patterns by means of pulse field gel electrophoresis (PFGE). Results show that (i) Sfi I restriction fragment patterns of genomic DNA of strain K-12(wild type) and its derivatives(either lysogenic
基金This research was supported by grants from New England Biolacs, Inc., USA.
文摘A new type Ⅱ restriction endonuclease, BsrG I, has been isolated from Bacillusstearothermophilus GR75. BsrG I cleaves T7 DNA at 13 sites, Lambda DNA at
基金This research was supported by grants from New England BioLabs, Inc., USA.
文摘A new type Ⅱ restriction endonuclease, BsaO Ⅰ, has been isolated from the thermophilic bacillus Bacillus stearothermophilus O-122. This enzyme cleaves pBR322 DNA at 7 sites, pUC19 DNA at 5 sites and φ X 174 DNA at one site. The recognition sequence of BsaO Ⅰ was determined by mapping its cleavage sites on φ X 174 DNA to position 4600 and
文摘When blood DNA was digested with Taq I and monitored for RFLP with a human cDNA probe from the 21-hydroxylase genes (pC21/3c)in our laboratory, besides two characteristic restriction fragments 3.2 kb and 3.7 kb, an extra 5.6 kb band was revealed. This extra band appears both in normal Chinese individuals and in patients with congenital adrenal hyperplasia (CAH). The frequency is high (1/40 in phenotypically normal individuals and 1/18 in CAH patients). Evidently there is no selective pressure which is exerted
